Cloned (Comment) | Organism |
---|---|
gene Sephs2, recombinant expression of C-terminally His6-tagged enzyme mutant Sephs2U60C in Escherichia coli Rosetta (DE3) pLysS | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Sec60C | site-directed mutagenesis, the in-frame opal codon for Sec60 is altered to Cys-coding TGT in mutant Sephs2U60C, the substitution of active site Sec with Cys does not significantly perturb the catalytic function of the His6-tagged human Sephs2 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics for the C-terminally His6-tagged enzyme mutant Sephs2-U60C | Homo sapiens | |
0.026 | - |
ATP | pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | Homo sapiens | - |
AMP + selenophosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99611 | gene Sephs2 | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme mutant Sephs2U60C from Escherichia coli Rosetta (DE3) pLysS by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + selenide + H2O | - |
Homo sapiens | AMP + selenophosphate + phosphate | - |
? | |
additional information | development of a non-radioactive assay for detection of selenophosphate synthetase activity using recombinant pyruvate diphosphate dikinase, PPDK, from Thermus thermophilus strain HB8 in an enzyme-coupled reaction system, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sephs2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
ATP | pH 7.0, temperature not specified in the publication, recombinant His6-tagged enzyme mutant Sephs2-U60C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |