Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
thermophilic iron-oxidizing bacterium strain TI-1 | |
o-phenanthroline | - |
thermophilic iron-oxidizing bacterium strain TI-1 | |
Sodium cyanide | 2.5fold increase at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzene sufonic acid | strong inhibition at 0.1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
Co2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
Ni2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 | |
Zn2+ | slight inhibition at 1 mM | thermophilic iron-oxidizing bacterium strain TI-1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.125 | - |
NADH | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 | |
0.29 | - |
thiosulfate | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 | |
5 | - |
NADPH | pH 6, 45°C | thermophilic iron-oxidizing bacterium strain TI-1 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
thermophilic iron-oxidizing bacterium strain TI-1 | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
homotetramer, 4 * 58000, SDS-PAGE | thermophilic iron-oxidizing bacterium strain TI-1 |
243000 | - |
gel filtration | thermophilic iron-oxidizing bacterium strain TI-1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
thermophilic iron-oxidizing bacterium strain TI-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
thermophilic iron-oxidizing bacterium strain TI-1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiosulfate + NAD(P)H | - |
thermophilic iron-oxidizing bacterium strain TI-1 | H2S + NAD(P)+ + sulfite | - |
? | |
thiosulfate + NADH | - |
thermophilic iron-oxidizing bacterium strain TI-1 | H2S + NAD+ + sulfite | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | homotetramer, 4 * 58000, SDS-PAGE | thermophilic iron-oxidizing bacterium strain TI-1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
thermophilic iron-oxidizing bacterium strain TI-1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
no enzyme activity after 15 min | thermophilic iron-oxidizing bacterium strain TI-1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
thermophilic iron-oxidizing bacterium strain TI-1 |