Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | increase of enzyme activity at 50 mM | Synechocystis sp. | |
pyruvate | increase of enzyme activity at 5 mM | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
- |
Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
0.43 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. | |
0.59 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
17 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | [Fe2-S2]ferredoxin | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Synechocystis sp. |
87000 | - |
isoenzyme SsCsd1, gel filtration | Synechocystis sp. |
88000 | - |
isoenzyme SsCsd2, gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + [enzyme]-cysteine | Synechocystis sp. | - |
L-alanine + [enzyme]-S-sulfanylcysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
PCC6803 | - |
Purification (Comment) | Organism |
---|---|
isoenzyme SsCsd1, isoenzyme SsCsd2 and isoenzyme SsCsd3 | Synechocystis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
isoenzyme SsCsd1, with L-cysteine sulfinic acid as substrate | Synechocystis sp. |
4.8 | - |
isoenzyme SsCsd2, with L-cysteine sulfinic acid as substrate | Synechocystis sp. |
5.8 | - |
isoenzyme SsCsd2, with L-selenocysteine as substrate | Synechocystis sp. |
21 | - |
isoenzyme SsCsd1, with L-selenocysteine as substrate | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + [enzyme]-cysteine | - |
Synechocystis sp. | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
L-cysteine + [enzyme]-cysteine | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
L-cysteine sulfinic acid | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + sulfite | - |
? | |
L-cystine | - |
Synechocystis sp. | ? | - |
? | |
L-selenocysteine | L-cysteine desulfuration requires a cysteine residue at the active site of the enzyme, but decomposition of L-selenocysteine and L-cysteine sufinic acid do not | Synechocystis sp. | L-alanine + selenium | - |
? | |
L-selenocystine | - |
Synechocystis sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechocystis sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. | |
3.4 | - |
L-cysteine sulfinic acid | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
4 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd2 | Synechocystis sp. | |
15 | - |
L-selenocysteine | pH 7.5, 37°C, isoenzyme SsCsd1 | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isoenzyme SsCsd1 with L-selenocysteine and isoenzyme SsCsd2 with L-selenocysteine and L-cysteine sulfinic acid as substrates | Synechocystis sp. |
7.7 | - |
isoenzyme SsCsd1 with S-cysteine sulfinic acid as substrate | Synechocystis sp. |