Literature summary for 2.8.2.21 extracted from

Keller, R.; Driesch, R.; Stein, T.; Momburg, M.; Stuhlsatz, H.W.; Greiling, H.
Biosynthesis of proteokeratan sulfate in the bovine cornea. 1. Isolation and characterization of a keratan sulfotransferase and the role of sulfation for the chain termination (1983), Hoppe-Seyler's Z. Physiol. Chem., 364, 239-252.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2'-AMP	weak, above 0.08 mM	Bos taurus
3'-AMP	weak, above 0.08 mM	Bos taurus
5'-adenylylsulfate	-	Bos taurus
5'-AMP	weak, above 0.08 mM	Bos taurus
ADP	above 0.08 mM	Bos taurus
AMP	above 0.08 mM	Bos taurus
ATP	above 0.08 mM	Bos taurus
cAMP	weak	Bos taurus
Cu2+	above 1 mM, complete inhibition above 2 mM	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.025	-	Keratan sulfate	pH 6, 15°C	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Bos taurus	5829	-
microsome	-	Bos taurus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates, optimal concentration: 5 mM	Bos taurus
Co2+	activates, optimal concentration: 5 mM	Bos taurus
Mn2+	activates, optimal concentration: 5 mM	Bos taurus
Zn2+	activates, optimal concentration: 5 mM	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
240000	-	gel filtration	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
244fold	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cornea	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.000013	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylylsulfate + keratan sulfate	activity decreases with increasing molecular mass and sulfation degree of keratan sulfate	Bos taurus	adenosine 3',5'-bisphosphate + keratan 6'-sulfate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
12	-	-	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	2 optima: pH 6.0 and 8.6	Bos taurus
8.6	-	2 optima: pH 6.0 and 8.6	Bos taurus

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Release 2023.1 (January 2023)