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Literature summary for 2.8.2.30 extracted from
- Biophysical investigation of human heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A: a mutual effect of enzyme oligomerisation and glycosaminoglycan ligand binding (2007), Biochim. Biophys. Acta, 1774, 1470-1476.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(DE3)pLysS | Homo sapiens |
| expression of the N-terminally His6-tagged truncated mutant isozyme 3-OST-3A lacking the cytoplasmic, transmembrane and SPLAG domain | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a truncated version of 3-OST-3A starting at amino acid position G148, thereby lacking the cytoplasmic, transmembrane and SPLAG domain | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE | Homo sapiens |
| 33743 | - |
x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heparan sulfate + 3'-phosphoadenylyl sulfate | Homo sapiens | i.e. PAPS | 3-O-sulfated heparan sulfate + adenosine 3',5'-bisphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Co2+ affinity chromatography and heparin-agarose column chromatography | Homo sapiens |
| recombinant N-terminally His6-tagged truncated mutant isozyme 3-OST-3A lacking the cytoplasmic, transmembrane and SPLAG domain from Escherichia coli by cobalt and heparin affinity chromatography, followed by dialysis, to over 95% puritiy | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| addition of 6 M guanidinium-HCl results in a red shift of the emission maximum by 12 nm indicating that the enzyme is properly folded after purification | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-phosphoadenylyl sulfate + chondroitin sulfate | chondroitin sulfate exhibits similar high affinity binding to 3-OST-3A compared to heparan sulfate | Homo sapiens | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + heparin | heparin exhibits similar high affinity binding to 3-OST-3A compared to heparan sulfate | Homo sapiens | ? | - |
? | |
| 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine | 3-OST-3A generates a unique heparan sulfate sequence that is predicted to specifically bind envelope glycoprotein D of Herpes Simplex virus type 1, but not antithrombin III | Homo sapiens | adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate | - |
? | |
| heparan sulfate + 3'-phosphoadenylyl sulfate | i.e. PAPS | Homo sapiens | 3-O-sulfated heparan sulfate + adenosine 3',5'-bisphosphate | - |
? | |
| additional information | binding to different glycosaminoglycans, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE | Homo sapiens |
| More | analysis of the overall structural integrity of recombinant 3-OST-3A by steady-state fluorescence spectroscopy, secondary structure with a content of 38.7% alpha-helix and 13.3% beta-sheet, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-O-sulfotransferase-3A | - |
Homo sapiens |
| 3-OST-3A | - |
Homo sapiens |
| heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A | - |
Homo sapiens |
| heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A | the enzyme generates a binding site for the envelope glycoprotein D of herpes simplex virus 1 | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal unfolding kinetics of the purified recombinant His6-tagged truncated mutant protein, overview | Homo sapiens |
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