Application | Comment | Organism |
---|---|---|
synthesis | 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae are expressed in Escherichia coli and successfully used for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
co-expression of the enzyme with myrosinase from Brevicoryne brassicae in Escherichia coli strain BL21(DE3), expression of codon-optimized sulfotransferase gene SOT18 from Arabidopsis thaliana is successful and can substitute for the insoluble SOT16, desulfoglucosinolate:PAPS sulfotransferase | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and functionally expressed in Escherichia coli. The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli strain BL21(DE3). Reconstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT18, and MYR, respectively, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C9C9 | - |
- |
Arabidopsis thaliana Col-0 | Q9C9C9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies | Arabidopsis thaliana | ? | - |
- |
|
additional information | usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies | Arabidopsis thaliana Col-0 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase | UniProt | Arabidopsis thaliana |
cytosolic sulfotransferase 18 | - |
Arabidopsis thaliana |
SOT18 | - |
Arabidopsis thaliana |