Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
expression in Escherichia coli | Pseudomonas aeruginosa PAO1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Pseudomonas aeruginosa PAO1 | |
CoCl2 | 1mM, 34% residual activity | Pseudomonas aeruginosa | |
Cu2+ | - |
Pseudomonas aeruginosa PAO1 | |
CuCl2 | 1 mM, 59% residual activity | Pseudomonas aeruginosa | |
dithiothreitol | 1 mM, 58% residual activity | Pseudomonas aeruginosa | |
DTT | 1 mM, 42% inhibition | Pseudomonas aeruginosa PAO1 | |
Fe2+ | - |
Pseudomonas aeruginosa PAO1 | |
Fe3+ | - |
Pseudomonas aeruginosa PAO1 | |
FeCl2 | 1 mM, 40% residual activity | Pseudomonas aeruginosa | |
FeCl3 | 1 mM, 38% residual activity | Pseudomonas aeruginosa | |
additional information | not inhibitory: EDTA | Pseudomonas aeruginosa | |
additional information | no inhibition by EDTA | Pseudomonas aeruginosa PAO1 | |
Ni2+ | - |
Pseudomonas aeruginosa PAO1 | |
NiCl2 | 1 mM, 35% residual activity | Pseudomonas aeruginosa | |
phenylmethylsulfonyl fluoride | 0.1 mM, 48% residual activity | Pseudomonas aeruginosa | |
phenylmethylsulfonyl fluoride | complete inhibition above 1 mM | Pseudomonas aeruginosa PAO1 | |
Zn2+ | - |
Pseudomonas aeruginosa PAO1 | |
ZnCl2 | 1 mM, 36% residual activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
p-nitrophenyl acetate | - |
Pseudomonas aeruginosa PAO1 | |
0.43 | - |
4-nitrophenyl acetate | pH 9.0, 25°C | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | slight activation | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19300 | - |
gel filtration | Pseudomonas aeruginosa |
19300 | - |
gel filtration | Pseudomonas aeruginosa PAO1 |
24000 | - |
1 * 24000, SDS-PAGE | Pseudomonas aeruginosa PAO1 |
24691 | - |
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS | Pseudomonas aeruginosa |
28000 | - |
1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa PAO1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Pseudomonas aeruginosa PAO1 |
recombinant protein | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7310 | - |
25°C, pH 7.0 | Pseudomonas aeruginosa PAO1 |
7310 | - |
pH 9.0, 25°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Pseudomonas aeruginosa | 1-naphthol + acetate | - |
? | |
1-naphthyl butanoate + H2O | 54% of the activity with 1-naphthyl acetate | Pseudomonas aeruginosa | 1-naphthol + butanoate | - |
? | |
1-naphthyl caprylate + H2O | 14% of the activity with 1-naphthyl acetate | Pseudomonas aeruginosa | 1-naphthol + caprylate | - |
? | |
1-naphthyl laurate + H2O | 9% of the activity with 1-naphthyl acetate | Pseudomonas aeruginosa | 1-naphthol + laurate | - |
? | |
1-naphthyl oleate + H2O | 2% of the activity with 1-naphthyl acetate | Pseudomonas aeruginosa | 1-naphthol + oleate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Pseudomonas aeruginosa | 4-methylumbelliferone + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Pseudomonas aeruginosa PAO1 | 4-methylumbelliferone + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Pseudomonas aeruginosa | 4-nitrophenol + butanoate | - |
? | |
alpha-naphthyl acetate + H2O | - |
Pseudomonas aeruginosa PAO1 | alpha-naphthol + acetate | - |
? | |
alpha-naphthyl butyrate + H2O | - |
Pseudomonas aeruginosa PAO1 | alpha-naphthol + butyrate | - |
? | |
carboxyfluorescein diacetate + H2O | - |
Pseudomonas aeruginosa | carboxyfluorescein + acetate | - |
? | |
carboxyfluorescein diacetate + H2O | - |
Pseudomonas aeruginosa PAO1 | ? | - |
? | |
fluorescein diacetate + H2O | - |
Pseudomonas aeruginosa PAO1 | ? | - |
? | |
fluorescein diacetate + H2O | - |
Pseudomonas aeruginosa | fluorescein + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Pseudomonas aeruginosa PAO1 | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate | - |
Pseudomonas aeruginosa PAO1 | p-nitrophenol + butyrate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE | Pseudomonas aeruginosa PAO1 |
monomer | 1 * 28000, SDS-PAGE, 1 * 24691, ESI-MS | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Pseudomonas aeruginosa |
55 | - |
- |
Pseudomonas aeruginosa PAO1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | 45°C: about 60% of maximal activity, 65°C: about 50% of maximal activity | Pseudomonas aeruginosa PAO1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 9, 2 h, stable up to | Pseudomonas aeruginosa PAO1 |
50 | - |
pH 9.0, 2 h, stable up to 50°C | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
fluorescein diacetate | - |
Pseudomonas aeruginosa | |
170 | - |
fluorescein diacetate | - |
Pseudomonas aeruginosa PAO1 | |
1600 | - |
1-naphthyl butanoate | - |
Pseudomonas aeruginosa | |
1600 | - |
alpha-naphthyl butyrate | - |
Pseudomonas aeruginosa PAO1 | |
1700 | - |
p-nitrophenyl butyrate | - |
Pseudomonas aeruginosa PAO1 | |
1700 | - |
4-nitrophenyl butanoate | - |
Pseudomonas aeruginosa | |
2800 | - |
carboxyfluorescein diacetate | - |
Pseudomonas aeruginosa | |
2800 | - |
carboxyfluorescein diacetate | - |
Pseudomonas aeruginosa PAO1 | |
3000 | - |
Alpha-naphthyl acetate | - |
Pseudomonas aeruginosa PAO1 | |
3000 | - |
1-naphthyl acetate | - |
Pseudomonas aeruginosa | |
6400 | - |
4-nitrophenyl acetate | - |
Pseudomonas aeruginosa | |
6400 | - |
p-nitrophenyl acetate | - |
Pseudomonas aeruginosa PAO1 | |
8500 | - |
4-yethylumbelliferyl acetate | - |
Pseudomonas aeruginosa | |
8500 | - |
4-yethylumbelliferyl acetate | - |
Pseudomonas aeruginosa PAO1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas aeruginosa |
9 | - |
- |
Pseudomonas aeruginosa PAO1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | about 40% of maximal activity at pH 8.0 and 10.0 | Pseudomonas aeruginosa PAO1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | stable | Pseudomonas aeruginosa PAO1 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | calculated | - |
5 |
Pseudomonas aeruginosa | isoelectric focusing | - |
5.2 |
Pseudomonas aeruginosa PAO1 | isoelectric focusing, pH-range 3-10 | - |
5.2 |