Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, Est55, 33% stimulation, Est30, 7% stimulation | Geobacillus stearothermophilus | |
dithiothreitol | 1 mM, Est55, 45% stimulation, Est30, 719% stimulation | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
p-nitrophenyl caproate | Est55, pH 8.5, 60°C | Geobacillus stearothermophilus | |
0.0022 | - |
p-nitrophenyl caproate | Est30, pH 8.5, 60°C | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28338 | - |
Est30, 2 * 28338, calculated | Geobacillus stearothermophilus |
46000 | - |
Est55, gel filtration | Geobacillus stearothermophilus |
54867 | - |
Est55, 1 * 54867, calculated | Geobacillus stearothermophilus |
55000 | - |
Est30, gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
isoforms Est30, Est55 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | Est55, 30% of the activity with 4-nitrophenyl butanoate, Est30, 20% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | Est30, 65% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl caprate + H2O | Est55, 1.6% of the activity with 4-nitrophenyl butanoate, Est30, 32% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + capric acid | - |
? | |
4-nitrophenyl caproate + H2O | Est55, 97% of the activity with 4-nitrophenyl butanoate, activation energy 35.7 kJ/mol. Est30, activation energy 101.9 kJ/mol | Geobacillus stearothermophilus | 4-nitrophenol + caproate | - |
? | |
4-nitrophenyl caprylate + H2O | Est55, 13% of the activity with 4-nitrophenyl butanoate, Est30, 50% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | Est55, 0.6% of the activity with 4-nitrophenyl butanoate, Est30, 12% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + lauric acid | - |
? | |
4-nitrophenyl propanoate + H2O | Est55, 50% of the activity with 4-nitrophenyl butanoate, Est30, 45% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + propanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | Est30, 2 * 28338, calculated | Geobacillus stearothermophilus |
monomer | Est55, 1 * 54867, calculated | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
Est30 | - |
Geobacillus stearothermophilus |
Est55 | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
Est55 | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for more than 2 h, Est55 and Est30 | Geobacillus stearothermophilus |
70 | - |
Est55, half-life 40 min, Est30, half-life 180 min | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
p-nitrophenyl caproate | Est30, pH 8.5, 60°C | Geobacillus stearothermophilus | |
39760 | - |
p-nitrophenyl caproate | Est55, pH 8.5, 60°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | broad, Est55 | Geobacillus stearothermophilus |
9 | - |
Est30 | Geobacillus stearothermophilus |