Cloned (Comment) | Organism |
---|---|
expressed as GFP-fusion protein in host strain, overexpression of PME results in reduced pollen tube growth | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Nicotiana tabacum | 5783 | - |
Golgi stack | - |
Nicotiana tabacum | 5795 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Nicotiana tabacum |
60300 | - |
x * 60300, calculated from the deduced amino acid sequence of the pre-pro-protein | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | Nicotiana tabacum | involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth | methanol + pectate | - |
? | |
pectin + H2O | Lilium formosanum | involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth | methanol + pectate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lilium formosanum | - |
cells cultured in the presence of PME from orange peel | - |
Nicotiana tabacum | - |
cells cultured in the presence of PME from orange peel | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pollen | - |
Nicotiana tabacum | - |
pollen | - |
Lilium formosanum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Nicotiana tabacum | methanol + pectate | - |
? | |
pectin + H2O | - |
Lilium formosanum | methanol + pectate | - |
? | |
pectin + H2O | involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth | Nicotiana tabacum | methanol + pectate | - |
? | |
pectin + H2O | involved in the regulation of the cell wall rigidity, application of exogenous PME causes thickening of the apical cell wall and inhibits pollen tube growth | Lilium formosanum | methanol + pectate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Nicotiana tabacum |
? | x * 39000-41500, calculated from the deduced amino acid sequence for the mature protein | Nicotiana tabacum |
? | x * 60300, calculated from the deduced amino acid sequence of the pre-pro-protein | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
Nicotiana tabacum pollen tube PME1 | expression of the described pre-pro-PME is restricted to pollen and pollen tubes | Nicotiana tabacum |
NtPPME1 | - |
Nicotiana tabacum |
pectin methylesterase | - |
Nicotiana tabacum |
pectin methylesterase | - |
Lilium formosanum |
PME | - |
Nicotiana tabacum |
PME | - |
Lilium formosanum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nicotiana tabacum | isoelectric focusing | - |
8.5 |
Nicotiana tabacum | calculated from the deduced amino acid sequence for the mature protein | - |
8.6 |