Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 27% inhibition at 1 mM | Kluyveromyces marxianus | |
Co2+ | 32% inhibition at 1 mM | Kluyveromyces marxianus | |
Hg2+ | 57% inhibition at 1 mM | Kluyveromyces marxianus | |
K+ | 58% inhibition at 1 mM | Kluyveromyces marxianus | |
Mg2+ | 10% inhibition at 1 mM | Kluyveromyces marxianus | |
Mn2+ | 60% inhibition at 1 mM | Kluyveromyces marxianus | |
Na+ | 30% inhibition at 1 mM | Kluyveromyces marxianus | |
Zn2+ | 22% inhibition at 1 mM | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.77 | - |
Tannic acid | pH pH 5.5, 30°C | Kluyveromyces marxianus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 12% activation at 1 mM | Kluyveromyces marxianus | |
Cd2+ | 42.2% activation at 1 mM | Kluyveromyces marxianus | |
Cu2+ | 35.8% activation at 1 mM | Kluyveromyces marxianus | |
Fe3+ | 28.5% activation at 1 mM | Kluyveromyces marxianus | |
Li+ | 14% activation at 1 mM | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66620 | - |
gel filtration | Kluyveromyces marxianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Kluyveromyces marxianus | - |
gallic acid + ? | - |
? | |
tannic acid + H2O | Kluyveromyces marxianus NRRL Y-8281 | - |
gallic acid + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Kluyveromyces marxianus NRRL Y-8281 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 24.21fold by ammonium sulfate fractionation and gel filtration | Kluyveromyces marxianus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | culture growth on olive pomace as solid support in solid state fermentation | Kluyveromyces marxianus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1026.12 | - |
purified native enzyme, pH 5.5, 30°C | Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epigallocatechin gallate + H2O | - |
Kluyveromyces marxianus | gallic acid + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Kluyveromyces marxianus NRRL Y-8281 | gallic acid + epigallocatechin | - |
? | |
methyl gallate + H2O | - |
Kluyveromyces marxianus | methanol + gallic acid | - |
? | |
methyl gallate + H2O | - |
Kluyveromyces marxianus NRRL Y-8281 | methanol + gallic acid | - |
? | |
additional information | the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) | Kluyveromyces marxianus | ? | - |
? | |
additional information | the enzyme is most active on tannic acid (100%) followed by methyl gallate (74.3%) and propyl gallate (64.5%) showing minimum enzymatic activity toward epigallocatechin gallate (10.5%) | Kluyveromyces marxianus NRRL Y-8281 | ? | - |
? | |
propyl gallate + H2O | - |
Kluyveromyces marxianus | propanol + gallic acid | - |
? | |
propyl gallate + H2O | - |
Kluyveromyces marxianus NRRL Y-8281 | propanol + gallic acid | - |
? | |
tannic acid + H2O | - |
Kluyveromyces marxianus | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Kluyveromyces marxianus NRRL Y-8281 | gallic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Kluyveromyces marxianus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity profile overview | Kluyveromyces marxianus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | purified native enzyme, thermostable within the temperature range 30-70°C, 30 min | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
optimum 1 | Kluyveromyces marxianus |
8.5 | - |
optimum 2 | Kluyveromyces marxianus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity profile overview | Kluyveromyces marxianus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | purified native enzyme, stable in the pH range of pH 4.0-5.5 for 30 min | Kluyveromyces marxianus |