BRENDA - Enzyme Database show
show all sequences of 3.1.1.24

p-Chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl CoA: beta-ketoadipate CoA transferase

Yeh, W.K.; Ornston, L.N.; Arch. Microbiol. 138, 102-105 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-oxoadipate
50% inhibition at a concentration of 17 mM; i.e. beta-ketoadipate
Acinetobacter calcoaceticus
3-oxoadipate
10% inhibition at a concentration of 1 mM; 50% inhibition at a concentration of 10 mM; i.e. beta-ketoadipate
Pseudomonas putida
5,5'-dithiobis(2-nitrobenzoic acid)
completely inactivated, activity can not be recovered by adding dithiothreitol
Pseudomonas putida
additional information
not inhibited by adipate and levulinate at a concentration of 20 mM
Acinetobacter calcoaceticus
additional information
not inhibited by adipate and levulinate at a concentration of 20 mM
Pseudomonas putida
p-chloromercuribenzoate
-
Acinetobacter calcoaceticus
p-chloromercuribenzoate
completely inactivated at a concentration of 0.005 mM, 95% of the activity can be recovered by addition of 10 mM dithiothreitol in the reaction mixture; inhibition can not be prevented in the presence of beta-ketoadipate
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
Pseudomonas putida
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
?
-
-
-
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
Acinetobacter calcoaceticus
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
-
-
Pseudomonas putida
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
3-oxoadipate enol-lactone
94277
Pseudomonas putida
3-oxoadipate
-
-
-
?
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
3-oxoadipate enol-lactone
94277
Acinetobacter calcoaceticus
3-oxoadipate
-
-
-
?
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
94277
Pseudomonas putida
?
-
-
-
-
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
94277
Acinetobacter calcoaceticus
?
-
-
-
-
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10
-
3-oxoadipate
-
Pseudomonas putida
17
-
3-oxoadipate
-
Acinetobacter calcoaceticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-oxoadipate
50% inhibition at a concentration of 17 mM; i.e. beta-ketoadipate
Acinetobacter calcoaceticus
3-oxoadipate
10% inhibition at a concentration of 1 mM; 50% inhibition at a concentration of 10 mM; i.e. beta-ketoadipate
Pseudomonas putida
5,5'-dithiobis(2-nitrobenzoic acid)
completely inactivated, activity can not be recovered by adding dithiothreitol
Pseudomonas putida
additional information
not inhibited by adipate and levulinate at a concentration of 20 mM
Acinetobacter calcoaceticus
additional information
not inhibited by adipate and levulinate at a concentration of 20 mM
Pseudomonas putida
p-chloromercuribenzoate
-
Acinetobacter calcoaceticus
p-chloromercuribenzoate
completely inactivated at a concentration of 0.005 mM, 95% of the activity can be recovered by addition of 10 mM dithiothreitol in the reaction mixture; inhibition can not be prevented in the presence of beta-ketoadipate
Pseudomonas putida
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
10
-
3-oxoadipate
-
Pseudomonas putida
17
-
3-oxoadipate
-
Acinetobacter calcoaceticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
Pseudomonas putida
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
?
-
-
-
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
Acinetobacter calcoaceticus
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
3-oxoadipate enol-lactone
94277
Pseudomonas putida
3-oxoadipate
-
-
-
?
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
3-oxoadipate enol-lactone
94277
Acinetobacter calcoaceticus
3-oxoadipate
-
-
-
?
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
94277
Pseudomonas putida
?
-
-
-
-
(5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O
the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase
94277
Acinetobacter calcoaceticus
?
-
-
-
-
Other publictions for EC 3.1.1.24
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747316
Yamanashi
In vitro reconstitution of th ...
Rhodococcus jostii
Biosci. Biotechnol. Biochem.
79
830-835
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730909
Knapik
A multi-faceted analysis of Ru ...
Paraburkholderia xenovorans
Proteins
80
2359-2368
2012
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715929
Bains
A product analog bound form of ...
Paraburkholderia xenovorans
J. Mol. Biol.
406
649-658
2011
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704325
Marin
Modified 3-oxoadipate pathway ...
Pseudomonas reinekei
J. Bacteriol.
192
1543-1552
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
33102
Eulberg
Characterization of a protocat ...
Rhodococcus opacus
J. Bacteriol.
180
1072-1081
1998
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3671
Wong
-
Evidence for two uptake system ...
Rhizobium leguminosarum
Arch. Microbiol.
156
385-391
1991
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94275
Hughes
Cloning and expression of pca ...
Pseudomonas putida, Pseudomonas putida PRS2000
J. Gen. Microbiol.
134
2877-2887
1988
-
-
1
-
-
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2976
Shanley
Cloning and expression of Acin ...
Acinetobacter calcoaceticus
J. Bacteriol.
165
557-563
1986
-
-
1
-
-
-
1
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94277
Yeh
p-Chloromercuribenzoate specif ...
Acinetobacter calcoaceticus, Pseudomonas putida
Arch. Microbiol.
138
102-105
1984
-
-
-
-
-
-
7
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
7
2
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94278
Yeh
Evolutionary divergence of co- ...
Acinetobacter calcoaceticus
J. Biol. Chem.
255
6342-6346
1980
-
-
-
-
-
-
-
-
-
-
1
1
-
5
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94279
McCorkle
Repetitions in the NH2-termina ...
Pseudomonas putida
J. Biol. Chem.
255
6335-6341
1980
-
-
-
-
-
-
-
-
-
-
2
1
-
6
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
94280
Patel
beta-Ketoadipate enol-lactone ...
Acinetobacter calcoaceticus
J. Biol. Chem.
250
6567-6577
1975
-
-
-
-
-
-
-
-
-
-
2
1
-
5
-
-
1
-
-
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
2
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2972
Ornston
-
Conversion of catechol and pro ...
Pseudomonas putida
Methods Enzymol.
17A
529-549
1970
-
-
-
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3661
Ornston
The conversion of catechol and ...
Pseudomonas putida, Pseudomonas putida A.312
J. Biol. Chem.
241
3787-3794
1966
-
-
-
-
-
-
-
1
-
-
1
-
-
2
-
-
1
-
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-