Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, analysis, expression in Escherichia coli DH5alpha | Staphylococcus simulans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by bile salts or amphiphilic proteins | Staphylococcus simulans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme is secreted to the culture medium | Staphylococcus simulans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 1.6fold, not required for expression of the enzyme, optimal at 3 mM | Staphylococcus simulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, SDS-PAGE | Staphylococcus simulans |
158000 | - |
gel filtration | Staphylococcus simulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus simulans | Q84EK3 | - |
- |
Purification (Comment) | Organism |
---|---|
397fold, to homogeneity from culture supernatant | Staphylococcus simulans |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1100 | - |
purified enzyme, substrate tributyrin, 3 mM CaCl2 | Staphylococcus simulans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme hydrolyzes triacylglycerols without chain length specificity | Staphylococcus simulans | ? | - |
? | |
tributyrin + H2O | - |
Staphylococcus simulans | dibutyrin + butyrate | - |
? | |
triolein + H2O | - |
Staphylococcus simulans | diolein + oleate | - |
? | |
tripropionin + H2O | - |
Staphylococcus simulans | dipropionin + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Staphylococcus simulans |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Staphylococcus simulans |
SSL | - |
Staphylococcus simulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Staphylococcus simulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
inactivation after a few min | Staphylococcus simulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Staphylococcus simulans |