BRENDA - Enzyme Database show
show all sequences of 3.1.1.3

A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation

Rathi, P.; Bradoo, S.; Saxena, R.K.; Gupta, R.; Biotechnol. Lett. 22, 495-498 (2000)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Pseudomonas sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
conatians a hyper-thermostable lipase
-
Purification (Commentary)
Commentary
Organism
partial, 6.8fold
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
167
-
partially purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
castor oil + H2O
major acyl group is ricinoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
coconut oil + H2O
major acyl group is palmitic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
corn oil + H2O
major acyl groups are oleic acid and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
groundnut oil + H2O
major acyl group is oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
linseed oil + H2O
major acyl groups are oleic and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
mustard oil + H2O
major acyl groups are erucic and oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
neem oil + H2O
major acyl groups are palmitic, stearic and oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
olive oil + H2O
best substrate, major acyl group is oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
soybean oil + H2O
major acyl groups are oleic acid and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
sunflower oil + H2O
major acyl group is linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
8 h 100% stable, 13 h loss of 50% activity, after 2 h at 90°C 30% activation is observed
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
9
12
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
partial, 6.8fold
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
167
-
partially purified enzyme
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
castor oil + H2O
major acyl group is ricinoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
coconut oil + H2O
major acyl group is palmitic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
corn oil + H2O
major acyl groups are oleic acid and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
groundnut oil + H2O
major acyl group is oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
linseed oil + H2O
major acyl groups are oleic and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
mustard oil + H2O
major acyl groups are erucic and oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
neem oil + H2O
major acyl groups are palmitic, stearic and oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
olive oil + H2O
best substrate, major acyl group is oleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
soybean oil + H2O
major acyl groups are oleic acid and linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
sunflower oil + H2O
major acyl group is linoleic acid
650684
Pseudomonas sp.
?
-
650684
Pseudomonas sp.
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
8 h 100% stable, 13 h loss of 50% activity, after 2 h at 90°C 30% activation is observed
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
9
12
-
Pseudomonas sp.
Other publictions for EC 3.1.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)