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show all sequences of 3.1.1.3

Purification, refolding, and characterization of recombinant Pseudomonas fluorescens lipase

Kim, K.R.; Kwon, D.Y.; Yoon, S.H.; Kim, W.Y.; Kim, K.H.; Protein Expr. Purif. 39, 124-129 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli strain BL21 in inclusion bodies
Pseudomonas fluorescens
General Stability
General Stability
Organism
Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates the recombinant refolded enzyme, induces secondary structural changes, confers resistance to denaturation by guanidinium hydrochloride and to inactivation by DMSO and trifluoroethanol, Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas fluorescens
the enzyme is responsible for the spoilage of milk
?
-
-
-
additional information
Pseudomonas fluorescens SIK W1
the enzyme is responsible for the spoilage of milk
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas fluorescens
-
-
-
Pseudomonas fluorescens SIK W1
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant refolded enzyme 2.5fold from Escherichia coli strain BL21 by gel filtration and ion exchange chromatography
Pseudomonas fluorescens
Renatured (Commentary)
Commentary
Organism
solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9264
-
purified refolded recombinant enzyme
Pseudomonas fluorescens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is responsible for the spoilage of milk
666821
Pseudomonas fluorescens
?
-
-
-
-
additional information
the enzyme is responsible for the spoilage of milk
666821
Pseudomonas fluorescens SIK W1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
Ca2+ induces secondary structural changes, Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas fluorescens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli strain BL21 in inclusion bodies
Pseudomonas fluorescens
General Stability (protein specific)
General Stability
Organism
Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates the recombinant refolded enzyme, induces secondary structural changes, confers resistance to denaturation by guanidinium hydrochloride and to inactivation by DMSO and trifluoroethanol, Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Pseudomonas fluorescens
the enzyme is responsible for the spoilage of milk
?
-
-
-
additional information
Pseudomonas fluorescens SIK W1
the enzyme is responsible for the spoilage of milk
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant refolded enzyme 2.5fold from Escherichia coli strain BL21 by gel filtration and ion exchange chromatography
Pseudomonas fluorescens
Renatured (Commentary) (protein specific)
Commentary
Organism
solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
9264
-
purified refolded recombinant enzyme
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme is responsible for the spoilage of milk
666821
Pseudomonas fluorescens
?
-
-
-
-
additional information
the enzyme is responsible for the spoilage of milk
666821
Pseudomonas fluorescens SIK W1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
Ca2+ induces secondary structural changes, Ca2+ is important for structural stability of the enzyme
Pseudomonas fluorescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas fluorescens
Other publictions for EC 3.1.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)