Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropylfluorophosphate | 0.001 mM, 50% inhibition after 10 min preincubation at 37°C | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
ethanol | cosubstrate oleate | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fatty acid + ethanol | Rattus norvegicus | enzyme contributes to the nonoxidative ethanol metabolism in various organs | fatty acyl ethyl ester + H2O | - |
? | |
fatty acid + ethanol | Rattus norvegicus | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | fatty acyl ethyl ester + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar rats weighing 300-400 g | - |
Purification (Comment) | Organism |
---|---|
from adipose tissue, 2 enzyme forms | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
additional information | not: kidney | Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
0.015 | - |
pH 7, 37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fatty acid + ethanol | substrate specificity | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
fatty acid + ethanol | enzyme contributes to the nonoxidative ethanol metabolism in various organs | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
fatty acid + ethanol | production of a nonoxidative metabolite of ethanol that accumulates in vivo with potentially toxic effects | Rattus norvegicus | fatty acyl ethyl ester + H2O | - |
? | |
monoacylglycerol + H2O | hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety | Rattus norvegicus | fatty acid + glycerol | - |
? | |
additional information | FAEES is associated with carboxylesterase in adipose tissue | Rattus norvegicus | ? | - |
? | |
oleate + ethanol | ethyl oleate-hydrolyzing activity is about one-eighth of the synthesizing activity | Rattus norvegicus | ethyl oleate + H2O | - |
r | |
triacylglycerol + H2O | hydrolysis, susceptibilities of substrates increase with decreasing acyl chain length of the fatty acid moiety | Rattus norvegicus | ? | - |
? | |
triolein + H2O | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimum and assay at | Rattus norvegicus |