Cloned (Comment) | Organism |
---|---|
gene fae1A, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) | Anaeromyces mucronatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Fae1A is not affected by the addition of EDTA | Anaeromyces mucronatus | |
phenylmethylsulfonyl fluoride | - |
Anaeromyces mucronatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
ethyl ferulate | pH 7.2, 37°C | Anaeromyces mucronatus | |
0.0067 | - |
methyl ferulate | pH 7.2, 37°C | Anaeromyces mucronatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anaeromyces mucronatus | F2YCB6 | gene fae1A | - |
Anaeromyces mucronatus YE505 | F2YCB6 | gene fae1A | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-naphthyl acetate + H2O | - |
Anaeromyces mucronatus | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Anaeromyces mucronatus YE505 | 2-naphthol + acetate | - |
? | |
ethyl ferulate + H2O | - |
Anaeromyces mucronatus | ferulic acid + ethanol | - |
? | |
ethyl ferulate + H2O | - |
Anaeromyces mucronatus YE505 | ferulic acid + ethanol | - |
? | |
methyl ferulate + H2O | - |
Anaeromyces mucronatus | ferulic acid + methanol | - |
? | |
methyl ferulate + H2O | - |
Anaeromyces mucronatus YE505 | ferulic acid + methanol | - |
? | |
additional information | Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity | Anaeromyces mucronatus | ? | - |
? | |
additional information | Fae1A exhibist activity against a number of substrates including naphthyl fatty acid esters, 4nitrophenyl fatty acid esters and hydroxylcinnamic acid esters, it has a higher affinity for ethyl and methyl ferulate than for the acetyl esters. It releases ferulic acid and 4-coumaric acid from barley straw. A serine residue plays a role in its activity | Anaeromyces mucronatus YE505 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fae1A | - |
Anaeromyces mucronatus |
ferulic acid esterase | - |
Anaeromyces mucronatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Anaeromyces mucronatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 37 | activity is optimal at 37°C, but 60% of activity is retained at 15°C. No activity is observed at 45°C | Anaeromyces mucronatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Anaeromyces mucronatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | over 50% activity within this range | Anaeromyces mucronatus |