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Literature summary for 3.1.1.73 extracted from
- Expression, purification and characterization of a feruloyl esterase A from Aspergillus flavus (2013), Protein Expr. Purif., 92, 36-40.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the enzyme is a promising biocatalyst for biomass degradation | Aspergillus flavus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression in Pichia pastoris | Aspergillus flavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Aspergillus flavus | |
| 0.26 | - |
methyl sinapinate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus |  |
| 0.31 | - |
methyl 4-coumarate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
| 0.44 | - |
methyl ferulate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28164 | - |
x * 40000, recombinant enzyme, SDS-PAGE, x * 28164, sequence calculation | Aspergillus flavus |
| 40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE, x * 28164, sequence calculation | Aspergillus flavus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
type-A feruloyl esterase | - |
| Aspergillus flavus CBE332.1 | - |
type-A feruloyl esterase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Pichia pastoris | Aspergillus flavus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus flavus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl 4-coumarate + H2O | - |
Aspergillus flavus | 4-coumarate + methanol | - |
? | |
| methyl 4-coumarate + H2O | - |
Aspergillus flavus CBE332.1 | 4-coumarate + methanol | - |
? | |
| methyl caffeate + H2O | - |
Aspergillus flavus | caffeic acid + methanol | - |
? | |
| methyl caffeate + H2O | - |
Aspergillus flavus CBE332.1 | caffeic acid + methanol | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus flavus | ferulate + methanol | - |
? | |
| methyl ferulate + H2O | - |
Aspergillus flavus CBE332.1 | ferulate + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Aspergillus flavus | sinapate + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Aspergillus flavus CBE332.1 | sinapate + methanol | - |
? | |
| additional information | release ferulic acid from steam exploded corn stalk by the enzyme in concert with Geobacillus stearothermophilus xylanase mutant | Aspergillus flavus | ? | - |
? | |
| additional information | release ferulic acid from steam exploded corn stalk by the enzyme in concert with Geobacillus stearothermophilus xylanase mutant | Aspergillus flavus CBE332.1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, recombinant enzyme, SDS-PAGE, x * 28164, sequence calculation | Aspergillus flavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AfFaeA | - |
Aspergillus flavus |
| feruloyl esterase A | - |
Aspergillus flavus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
recombinant enzyme | Aspergillus flavus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 65 | activity range, overview | Aspergillus flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, pH 4.5-8.0, 60 min, stable | Aspergillus flavus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
methyl 4-coumarate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
| 0.9 | - |
methyl sinapinate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
| 2.21 | - |
methyl ferulate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme | Aspergillus flavus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | 90% of maximum activity within this range | Aspergillus flavus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | purified recombinant enzyme, 60 min, 40°C, stable at | Aspergillus flavus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.88 | - |
methyl 4-coumarate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
| 3.48 | - |
methyl sinapinate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
| 5.02 | - |
methyl ferulate | pH 5.5, 40°C, recombinant enzyme | Aspergillus flavus | |
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