Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Fusarium oxysporum |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure and docking of substrates | Fusarium oxysporum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | introduction of mutations to provide hydrophobic environment for increased hydrolysis of sinapate substrates | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.331 | - |
methyl ferulate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
0.424 | - |
methyl sinapate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
0.649 | - |
methyl caffeate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
0.799 | - |
methyl p-coumarate | wild-type, pH 6.0, 37°C | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | A0A1D3S5H0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl caffeate + H2O | 85% of the activity with methyl p-coumarate | Fusarium oxysporum | methanol + caffeate | - |
? | |
methyl ferulate + H2O | 54% of the activity with methyl p-coumarate | Fusarium oxysporum | methanol + ferulate | - |
? | |
methyl p-coumarate + H2O | - |
Fusarium oxysporum | methanol + p-coumarate | - |
? | |
methyl sinapate + H2O | 2.8% of the activity with methyl p-coumarate | Fusarium oxysporum | methanol + sinapate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FaeC | - |
Fusarium oxysporum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
methyl sinapate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
232 | - |
methyl ferulate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
438 | - |
methyl caffeate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
546 | - |
methyl p-coumarate | wild-type, pH 6.0, 37°C | Fusarium oxysporum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
methyl sinapate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
675 | - |
methyl caffeate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
682 | - |
methyl p-coumarate | wild-type, pH 6.0, 37°C | Fusarium oxysporum | |
744 | - |
methyl ferulate | wild-type, pH 6.0, 37°C | Fusarium oxysporum |