Any feedback?
Literature summary for 3.1.2.12 extracted from
- A role for His-160 in peroxide inhibition of S. cerevisiae S-formylglutathione hydrolase: evidence for an oxidation sensitive motif (2012), Arch. Biochem. Biophys., 528, 7-20.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C60A | Km and kcat(4-nitrophenyl acetate) slightly decreased compared to wild-type | Saccharomyces cerevisiae |
| C60S | Km and kcat(4-nitrophenyl acetate) slightly decreased compared to wild-type. mutant is not inactivated by peroxide | Saccharomyces cerevisiae |
| H160I | Oxidation leads to activation of mutant enzyme. Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Oxidized form (with or without catalase): Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) increased compared to wild-type | Saccharomyces cerevisiae |
| H160S | Km (4-nitrophenyl acetate) slightly decreased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type | Saccharomyces cerevisiae |
| N64A | Km (4-nitrophenyl acetate) slightly increased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type | Saccharomyces cerevisiae |
| W197I | Km (4-nitrophenyl acetate) decreased and kcat (4-nitrophenyl acetate) decreased compared to wild-type | Saccharomyces cerevisiae |
| W197I/C60S | Km (4-nitrophenyl acetate) decreased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Km (S-lactolylglutathione) increased and kcat (4-nitrophenyl acetate) highly decreased compared to wild-type | Saccharomyces cerevisiae |
| W197I/H160I | Km (4-nitrophenyl acetate) increased and kcat (4-nitrophenyl acetate) decreased compared to wild-type. Km (S-lactolylglutathione) decreased and kcat (4-nitrophenyl acetate) highly decreased compared to wild-type | Saccharomyces cerevisiae |
| Y278F | Km (4-nitrophenyl acetate) slightly decreased and kcat (4-nitrophenyl acetate) slightly decreased compared to wild-type | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Saccharomyces cerevisiae |  |
| peroxide | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.054 | - |
4-nitrophenyl acetate | mutant W197I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.14 | - |
4-nitrophenyl acetate | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.16 | - |
4-nitrophenyl acetate | mutant H160S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.18 | - |
4-nitrophenyl acetate | mutant Y278F, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.19 | - |
4-nitrophenyl acetate | mutant C60A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.19 | - |
4-nitrophenyl acetate | mutant C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.4 | - |
4-nitrophenyl acetate | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.78 | - |
4-nitrophenyl acetate | mutant N64A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.9 | - |
S-Lactoylglutathione | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.99 | - |
4-nitrophenyl acetate | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 2.5 | - |
4-nitrophenyl acetate | mutant H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 2.6 | - |
4-nitrophenyl acetate | mutant H160I oxidized form with catalase, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 3 | - |
S-Lactoylglutathione | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 3 | - |
S-Lactoylglutathione | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 3.5 | - |
4-nitrophenyl acetate | mutant H160I oxidized form, pH 7.4, 22°C | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Saccharomyces cerevisiae | 4-nitrophenol + acetate | - |
? | |
| S-lactoylglutathione + H2O | - |
Saccharomyces cerevisiae | glutathione + lactate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S-formylglutathione hydrolase | - |
Saccharomyces cerevisiae |
| SFGH | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
4-nitrophenyl acetate | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.118 | - |
S-Lactoylglutathione | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.48 | - |
4-nitrophenyl acetate | mutant H160S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.6 | - |
4-nitrophenyl acetate | mutant Y278F, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.76 | - |
4-nitrophenyl acetate | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.85 | - |
4-nitrophenyl acetate | mutant H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.98 | - |
4-nitrophenyl acetate | mutant C60A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.05 | - |
4-nitrophenyl acetate | mutant W197I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.1 | - |
4-nitrophenyl acetate | mutant N64A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.5 | - |
4-nitrophenyl acetate | mutant C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.8 | - |
4-nitrophenyl acetate | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 2.8 | - |
4-nitrophenyl acetate | mutant H160I oxidized form with catalase, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 3.1 | - |
4-nitrophenyl acetate | mutant H160I oxidized form, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 50 | - |
S-Lactoylglutathione | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 116 | - |
S-Lactoylglutathione | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
Cu2+ | wild-type, 22°C, pH 7.4 | Saccharomyces cerevisiae | |
| 8 | - |
peroxide | wild-type, 22°C, pH 7.4, in the presence of substrate, inactivation is 10fold faster | Saccharomyces cerevisiae | |
| 12 | - |
peroxide | wild-type, 22°C, pH 7.4, in the absence of substrate | Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
4-nitrophenyl acetate | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.13 | - |
S-Lactoylglutathione | mutant W197I/H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.33 | - |
4-nitrophenyl acetate | mutant H160I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 0.83 | - |
4-nitrophenyl acetate | mutant H160I oxidized form, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.16 | - |
4-nitrophenyl acetate | mutant H160I oxidized form with catalase, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 1.41 | - |
4-nitrophenyl acetate | mutant N64A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 2.83 | - |
4-nitrophenyl acetate | mutant H160S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 3.33 | - |
4-nitrophenyl acetate | mutant Y278F, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 4.5 | - |
4-nitrophenyl acetate | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 5.16 | - |
4-nitrophenyl acetate | mutant C60A, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 5.5 | - |
4-nitrophenyl acetate | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 8 | - |
4-nitrophenyl acetate | mutant C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 16.6 | - |
S-Lactoylglutathione | mutant W197I/C60S, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 20 | - |
4-nitrophenyl acetate | mutant W197I, pH 7.4, 22°C | Saccharomyces cerevisiae | |
| 33.3 | - |
S-Lactoylglutathione | wild-type, pH 7.4, 22°C | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
