Literature summary for 3.1.2.6 extracted from

Rai, S.; Yadav, S.; Rai, R.; Chatterjee, A.; Singh, S.; Rai, L.C.
Molecular and biochemical characterization of All0580 as a methylglyoxal detoxifying glyoxalase II of Anabaena sp. PCC7120 that confers abiotic stress tolerance in E. coli (2019), Int. J. Biol. Macromol., 124, 981-993 .

Search for more literature for 3.1.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 DELTAgloB cells	Nostoc sp. PCC 7120 = FACHB-418

Inhibitors

Inhibitors	Comment	Organism	Structure
GSH	-	Nostoc sp. PCC 7120 = FACHB-418

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2982	-	S-D-lactoylglutathione	at pH 7.5 and 25°C	Nostoc sp. PCC 7120 = FACHB-418

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	the enzyme contains a binuclear Zn/Fe centre	Nostoc sp. PCC 7120 = FACHB-418
Zn2+	the enzyme contains a binuclear Zn/Fe centre	Nostoc sp. PCC 7120 = FACHB-418

Organism

Organism	UniProt	Comment	Textmining
Nostoc sp. PCC 7120 = FACHB-418	Q8YZ99	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography	Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-D-lactoylglutathione + H2O	-	Nostoc sp. PCC 7120 = FACHB-418	D-lactate + glutathione	-	?

Subunits

Subunits	Comment	Organism
?	x * 28700, SDS-PAGE	Nostoc sp. PCC 7120 = FACHB-418

Synonyms

Synonyms	Comment	Organism
All0580	-	Nostoc sp. PCC 7120 = FACHB-418
GLYII	-	Nostoc sp. PCC 7120 = FACHB-418
glyoxalase II	-	Nostoc sp. PCC 7120 = FACHB-418

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Nostoc sp. PCC 7120 = FACHB-418

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8	enhanced enzyme activity in the pH range of 6.5-8.0	Nostoc sp. PCC 7120 = FACHB-418

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2.5	-	at pH 7.5 and 25°C	Nostoc sp. PCC 7120 = FACHB-418	GSH

General Information

General Information	Comment	Organism
physiological function	the enzyme confers tolerance to abiotic stresses (salinity, drought, arsenic, heat, and UV-B stresses)	Nostoc sp. PCC 7120 = FACHB-418

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3000	-	S-D-lactoylglutathione	at pH 7.5 and 25°C	Nostoc sp. PCC 7120 = FACHB-418

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Release 2023.1 (January 2023)