Cloned (Comment) | Organism |
---|---|
expression in Spodoptera frugiperda Sf9 and Trichoplusia ni BTI-Tn-5B1-4 insect cells via infection with recombinant Autographa california multiple nuclear polyhedrovirus expressing SEAP under control of the polyhedrin promotor, the recombinant enzyme expressed in the 2 different insect cell lines show differing N-glycan structures | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.59 | - |
4-nitrophenyl phosphate | pH 9.8, recombinant enzyme expressed in Tn-5B1-4 cells | Homo sapiens | |
6.76 | - |
4-nitrophenyl phosphate | pH 9.8, recombinant enzyme expressed in Sf-9 cells | Homo sapiens | |
49.7 | - |
4-nitrophenyl phosphate | pH 9.8, native placental enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
1 * 64000, recombinant enzyme, SDS-PAGE, 1 * 66200, native enzyme, SDS-PAGE | Homo sapiens |
65000 | - |
about, recombinant enzyme, gel filtration | Homo sapiens |
66200 | - |
native placental enzyme, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
secreted alkaline phosphatase SEAP containing a single N-glycan chain, a model protein for investigation of glycosylation pattern | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | secreted alkaline phosphatase SEAP containing a single N-glycan chain, a model protein for investigation of glycosylation patterns, recombinant enzymes expressed in insect cells shows differing patterns: Sf-9 cell derived enzyme has Mna4GlcNAc2 and FucMan4GlcAc2 as major N-glycans, while the Tn-5B1-4 cell derived enzyme has Man2GlcAc2 as major N-glycan, glycan structures overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from expression in insect cells | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | placental SEAP | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
629 | - |
purified recombinant enzyme expressed in Sf-9 cells | Homo sapiens |
738 | - |
purified native placental enzyme | Homo sapiens |
850 | - |
purified recombinant enzyme expressed in Tn-5B1-4 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, recombinant enzyme, SDS-PAGE, 1 * 66200, native enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SEAP | - |
Homo sapiens |
secreted alkaline phosphatase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.8 | - |
assay at | Homo sapiens |