Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains BL21(lDE3) and DH5alpha | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants CNAaDELTAloop [containing the beta12-beta13 loop of PP1c in place of the L7 loop] and PP1DELTAloop [with the L7 loop (from Y311 to K318) substituted for the beta12-beta13 loop (from Y272 to A279)] show no activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activating effect at 2 mM | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.659 | - |
subunit CNAa-PP1-loop, crude cell lysate | Homo sapiens |
0.665 | - |
subunit PP1-CNAa-loop, crude cell lysate | Homo sapiens |
7.087 | - |
subunit CNAa-PP1-loop, after 10.8fold purification | Homo sapiens |
10.77 | - |
subunit PP1-CNAa-loop, after 16.2fold purification | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
myelin basic protein + H2O | - |
Homo sapiens | ? + phosphate | - |
? | |
phosphorylase a + H2O | - |
Homo sapiens | ? | - |
? | |
RII peptide + H2O | - |
Homo sapiens | ? + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calcineurin | - |
Homo sapiens |
CNAa | - |
Homo sapiens |
PP1 | - |
Homo sapiens |
PP2B | - |
Homo sapiens |
protein phosphatase 1 | - |
Homo sapiens |
protein phosphatase 2B | - |
Homo sapiens |
Ser/Thr protein phosphatase | - |
Homo sapiens |