Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 60% inhibition of PAP-2 | Rattus norvegicus | |
ceramide 1-phosphate | - |
Rattus norvegicus | |
chlorpromazine | 55% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
diacylglycerol | - |
Rattus norvegicus | |
F- | PAP-2 | Rattus norvegicus | |
lysophosphatidic acid | - |
Rattus norvegicus | |
Mg2+ | 15 mM MgCl2, 10-20% inhibition of PAP-2 | Rattus norvegicus | |
monoacylglycerol | - |
Rattus norvegicus | |
NEM | 2 mM, complete inhibition of PAP-1. NEM, up to 8 mM has little effect on PAP-2 | Rattus norvegicus | |
spermidine trihydrochloride | 7% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
spermine | 55% inhibition at equimolar concentration of substrate, PAP-2 | Rattus norvegicus | |
sphingomyelin | 72% inhibition at equimolar concentration with pure phosphatidic acid. No effect with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine | Rattus norvegicus | |
sphingosine | 93% inhibition at equimolar concentration with pure phosphatidic acid. 29% inhibition with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine | Rattus norvegicus | |
sphingosine 1-phosphate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | - |
Rattus norvegicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | PAP-1 is dependent on | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 different phosphatidate phosphatidases: PAP-1 and PAP-2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
alveolar cell type II | PAP-2 | Rattus norvegicus | - |
lung | PAP-2 | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lysophosphatidic acid + H2O | - |
Rattus norvegicus | ? | - |
? | |
additional information | key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis | Rattus norvegicus | ? | - |
? | |
phosphatidic acid + H2O | phosphatidic acid pure or in mixed micelles with phosphatidylcholine | Rattus norvegicus | 1,2-diacyl-sn-glycerol + phosphate | - |
? | |
sphingosine 1-phosphate + H2O | poor substrate | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP-1 | - |
Rattus norvegicus |
PAP-2 | - |
Rattus norvegicus |
PAPase | - |
Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
activity of PAP-2 is not seriously affected after 20 min | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
phosphatidic acid mixed with phosphatidylcholine, PAP-2 | Rattus norvegicus |
6.5 | - |
pure phosphatidic acid, PAP-2 | Rattus norvegicus |