Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rattus norvegicus | |
5'-nucleotides | - |
Rattus norvegicus | |
ADP | - |
Rattus norvegicus | |
AMP | 5'-AMP and slightly 3'-AMP | Rattus norvegicus | |
ATP | - |
Rattus norvegicus | |
Cu2+ | - |
Rattus norvegicus | |
CuCl2 | - |
Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
HgCl2 | - |
Rattus norvegicus | |
NAD+ | competitive | Rattus norvegicus | |
nucleoside 5'monophosphate | - |
Rattus norvegicus | |
nucleoside-5'-diphosphate | - |
Rattus norvegicus | |
p-chloromercuribenzoate | slight inhibition | Rattus norvegicus | |
RNA | competitive | Rattus norvegicus | |
terminal 3'-monophosphoryl group of substrates | - |
Rattus norvegicus | |
UDP | - |
Rattus norvegicus | |
UMP | 5'-UMP and very slightly 3'-UMP | Rattus norvegicus | |
UTP | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
4-nitrophenyl uridine 5'-phosphate | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation | Rattus norvegicus | |
Co2+ | slight activation | Rattus norvegicus | |
divalent cations | activation | Rattus norvegicus | |
K+ | activation with high concentration | Rattus norvegicus | |
KCl | activation | Rattus norvegicus | |
Mg2+ | activation | Rattus norvegicus | |
monovalent cations | activation with high concentrations | Rattus norvegicus | |
Na+ | activation with high concentration | Rattus norvegicus | |
NaCl | activation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.48 | - |
substrate: NAD+ | Rattus norvegicus |
1.2 | - |
substrate: p-nitrophenyluridine 5'-phosphate | Rattus norvegicus |
1.88 | - |
substrate: ApU | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl uridine 5'-phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + uridine 5'-phosphate | - |
? | |
5'-p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O | - |
Rattus norvegicus | ? | - |
? | |
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O | - |
Rattus norvegicus | nucleoside 5'-monophosphate + p-nitrophenol | - |
? | |
5'-p-nitrophenyluridine 5'-phosphate + H2O | - |
Rattus norvegicus | 5'-UMP + p-nitrophenol | - |
? | |
dinucleoside monophosphates + H2O | - |
Rattus norvegicus | mononucleoside 5'-phosphate | - |
? | |
DNA + H2O | heat-denatured: very slowly, native DNA is not hydrolyzed | Rattus norvegicus | DNA(n-1) + 2'-deoxynucleoside 5'-phosphate | - |
? | |
additional information | no activity with dinucleotides bearing 3'-phosphate | Rattus norvegicus | ? | - |
? | |
additional information | enzyme does not have a strict base specificity, it does not attack oligonucleotides with a 3'-phosphate terminal | Rattus norvegicus | ? | - |
? | |
additional information | no activity with UTP and UDP | Rattus norvegicus | ? | - |
? | |
NAD+ + H2O | - |
Rattus norvegicus | 5'-AMP + NMN | - |
? | |
oligonucleotides + H2O | specific for 3'-5'-direction from the 3'-hydroxyl terminal in a stepwise manner | Rattus norvegicus | oligonucleotides(n-1) + 5'-mononucleotides | - |
? | |
polymer synthesized from NAD+ + H2O | chain length 27-30 | Rattus norvegicus | ? | - |
? | |
polynucleotides + H2O | very slowly | Rattus norvegicus | polynucleotides(n-1) + 5'-mononucleotides | - |
? | |
RNA + H2O | very slowly | Rattus norvegicus | RNA(n-1) + nucleoside 5'-phosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is heat labile | Rattus norvegicus |
50 | - |
below, enzyme is heat labile | Rattus norvegicus |
70 | - |
pH 7.3, 5 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 10.5 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | - |
Rattus norvegicus |