General Stability | Organism |
---|---|
inactivation in absence of Mg2+ is aggravated at higher pH or when either EDTA or Ca2+ is added | Bacillus cereus |
repeated freezing and thawing causes rapid loss of activity | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | - |
Bacillus cereus | |
NaCl | - |
Bacillus cereus | |
phosphite | - |
Bacillus cereus | |
S2- | - |
Bacillus cereus | |
SO32- | - |
Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
phosphonoacetaldehyde | - |
Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | contains 0.3 gatom per molecular weight of 75000 Da | Bacillus cereus | |
Iron | contains trace amounts of iron | Bacillus cereus | |
Mg2+ | required | Bacillus cereus | |
Zinc | contains 0.4 gatom per molecular weight of 75000 Da | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
nondenaturing PAGE | Bacillus cereus |
83000 | - |
gel filtration | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonoacetylaldehyde + H2O | Bacillus cereus | second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid | acetaldehyde + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.9 | - |
- |
Bacillus cereus |
Storage Stability | Organism |
---|---|
-20°C, stable for several months | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenylphosphate + H2O | hydrolyzed at considerable lower rate than phosphonoacetaldehyde | Bacillus cereus | p-nitrophenol + phosphate | - |
? | |
phosphonoacetylaldehyde + H2O | 2-phosphonoacetaldehyde | Bacillus cereus | acetaldehyde + phosphate | - |
? | |
phosphonoacetylaldehyde + H2O | second step in the pathway by which Bacillus cereus metabolizes 2-aminoethylphosphonic acid | Bacillus cereus | acetaldehyde + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33000-37000, similar subunits, SDS-PAGE | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | 30°C: about 50% of maximal activity, 55°C: about 45% of maximal activity | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
rapid loss loss of activity in absence of Mg2+ | Bacillus cereus |
45 | - |
heat-labile, even in presence of 5 mM Mg2+ | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.7 | - |
2-phosphonoacetaldehyde | - |
Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | pH 6.5: about 35% of maximal activity, pH 10.0: about 30% of maximal activity | Bacillus cereus |