Any feedback?
Literature summary for 3.2.1.1 extracted from
- Production and characterization of raw-potato-starch-digesting alpha-amylase from Bacillus subtilis 65 (1988), Appl. Environ. Microbiol., 54, 1516-1522.
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Bacillus subtilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 2 mM, 61.2% inhibition | Bacillus subtilis |  |
| Al3+ | 2 mM, 38.7% inhibition | Bacillus subtilis | |
| Cd2+ | 2 mM, 43% inhibition | Bacillus subtilis | |
| Cu2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
| EDTA | 0.1 mM, 61.3% inhibition | Bacillus subtilis | |
| Fe3+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
| Hg2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
| Mn2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
| Pb2+ | 2 mM, 38% inhibition | Bacillus subtilis | |
| Zn2+ | 2 mM 30.3% inhibition | Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
amylose | - |
Bacillus subtilis | |
| 0.12 | - |
maltopentaose | - |
Bacillus subtilis | |
| 0.198 | - |
maltotriose | - |
Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 65 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.8 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylose + H2O | - |
Bacillus subtilis | additional information | - |
? | |
| glycogen + H2O | - |
Bacillus subtilis | additional information | - |
? | |
| maltopentaose + H2O | - |
Bacillus subtilis | additional information | - |
? | |
| maltotriose + H2O | - |
Bacillus subtilis | maltose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Bacillus subtilis 65 | maltose + D-glucose | - |
? | |
| p-nitrophenyl alpha-D-maltoside + H2O | - |
Bacillus subtilis | p-nitrophenol + maltose | - |
? | |
| starch + H2O | digestion of raw potato starch is almost as far as that of corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | sweet potato starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | waxy corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | wheat starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | digestion of raw potato starch is almost as far as that of corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | sweet potato starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
| starch + H2O | waxy corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | 40°C: about 30% of maximal activity, 80°C: about 40% of maximal activity | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, unstable above | Bacillus subtilis |
| 65 | - |
5 min, 95% loss of activity | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | pH 4.0: about 70% of maximal activity, pH 10.0: about 40% of maximal activity | Bacillus subtilis |
| 6 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 4°C, 24 h, stable | Bacillus subtilis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
