Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
L134R/S320A | the mutagenised protein is more acid resistant than the native protein. The optimum pH and stable range of pH with the mutagenised protein is 4.5 and 4.0 to 6.5, respectively, compared with pH 6.5 and 5.5 to 7.0 as the favorite pH and pH stability range of the native protein | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 23% inhibition of wild-type enzyme, 28% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Cd2+ | 1 mM, 33% inhibition of wild-type enzyme, 48% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Cu2+ | 1 mM, 55% inhibition of wild-type enzyme, 49% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Hg2+ | 1 mM, complete inhibition of wild-type enzyme and mutant enzyme L134R/S320A | Bacillus subtilis | |
Mg2+ | 1 mM, 11% inhibition of wild-type enzyme, 5% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Mn2+ | 1 mM, 20% inhibition of wild-type enzyme, 15% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Ni2+ | 1 mM, 23% inhibition of wild-type enzyme, 24% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis | |
Zn2+ | 1 mM, 28% inhibition of wild-type enzyme, 37% inhibition of mutant enzyme L134R/S320A | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.35fold activation of wild-type enzyme, 1.24fold activation of mutant enzyme L134R/S320A | Bacillus subtilis | |
Cs+ | 1 mM, 1.13fold activation of wild-type enzyme | Bacillus subtilis | |
Sr2+ | 1 mM, 1.11fold activation of wild-type enzyme, no activation of mutant enzyme L134R/S320A | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis WB600 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzyme L134R/S320A | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Bacillus subtilis | dextrin | - |
? | |
starch + H2O | - |
Bacillus subtilis WB600 | dextrin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
mutant enzyme L134R/S320A | Bacillus subtilis |
6.5 | - |
wild-type enzyme | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | pH 3.5: about 50% of maximal activity, pH 7.0: about 70% of maximal activity, mutant enzyme L134R/S320A | Bacillus subtilis |
5 | 7 | pH 5.0: about 70% of maximal activity, pH 7.0: about 90% of maximal activity, wild-type enzyme | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | 70°C, 1 h, stable pH-range for mutant enzyme L134R/S320A | Bacillus subtilis |
5.5 | 7 | 70°C, 1 h, stable pH-range for wild-type enzyme | Bacillus subtilis |