Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, T7 RNA polymerase expression system | Pyrococcus sp. |
General Stability | Organism |
---|---|
no loss of activity after dialysis of the purified enzyme overnight at 4°C against 100 mM MES buffer (pH 6.5) containing 1 mM EDTA | Pyrococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-cyclodextrin | 0.2%, 10% inhibition | Pyrococcus sp. | |
beta-cyclodextrin | 0.2%, 19% inhibition | Pyrococcus sp. | |
CoCl2 | 1 mM, 52% inhibition | Pyrococcus sp. | |
CuCl2 | 1 mM, complete inhibition | Pyrococcus sp. | |
gamma-cyclodextrin | 0.2%, 18% inhibition | Pyrococcus sp. | |
glucosyl-alpha-cyclodextrin | 0.2%, 19% inhibition | Pyrococcus sp. | |
MnCl2 | 1 mM, 55% inhibition | Pyrococcus sp. | |
SDS | 1.0% 63% inhibition | Pyrococcus sp. | |
ZnCl2 | 1 mM, complete inhibition | Pyrococcus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pyrococcus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Pyrococcus sp. |
49456 | - |
x * 49456, calculated from sequence | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | O33476 | - |
- |
Pyrococcus sp. KOD1 | O33476 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Pyrococcus sp. | ? | - |
? | |
amylopectin + H2O | - |
Pyrococcus sp. KOD1 | ? | - |
? | |
amylose + H2O | - |
Pyrococcus sp. | ? | - |
? | |
amylose + H2O | - |
Pyrococcus sp. KOD1 | ? | - |
? | |
glycogen + H2O | - |
Pyrococcus sp. | maltose + maltotriose | major products | ? | |
glycogen + H2O | - |
Pyrococcus sp. KOD1 | maltose + maltotriose | major products | ? | |
additional information | very weakly hydrolyzes pullulan to form panose or isopanose | Pyrococcus sp. | ? | - |
? | |
additional information | very weakly hydrolyzes pullulan to form panose or isopanose | Pyrococcus sp. KOD1 | ? | - |
? | |
starch + H2O | - |
Pyrococcus sp. | maltose + maltotriose | major products | ? | |
starch + H2O | - |
Pyrococcus sp. KOD1 | maltose + maltotriose | major products | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Pyrococcus sp. |
? | x * 49456, calculated from sequence | Pyrococcus sp. |
Synonyms | Comment | Organism |
---|---|---|
ApkA | - |
Pyrococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Pyrococcus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 60% of maximal activity, 100°C: about 80% of maximal activity | Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
60 min, about 10% loss of activity, thermostability is enhanced in the presence of 2 mM Ca2+ | Pyrococcus sp. |
100 | - |
60 min, in presence of 2 mM Ca2+, about 60% loss of activity | Pyrococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2280 | - |
amylose | pH 6.5, 90°C | Pyrococcus sp. | |
2300 | - |
glycogen | pH 6.5, 90°C | Pyrococcus sp. | |
4180 | - |
amylopectin | pH 6.5, 90°C | Pyrococcus sp. | |
4680 | - |
starch | pH 6.5, 90°C | Pyrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Pyrococcus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | pH 4.5: about 40% of maximal activity, pH 8.0: about 40% of maximal activity | Pyrococcus sp. |