Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli using the pCold I vector | Microbulbifer thermotolerans |
General Stability | Organism |
---|---|
the enzyme retains significant activity (50%) in the presence up to 2 M NaCl | Microbulbifer thermotolerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | 20% (v/v), 2 h, 20°C, 12% loss of activity | Microbulbifer thermotolerans | |
Benzene | 20% (v/v), 2 h, 20°C, 7% loss of activity | Microbulbifer thermotolerans | |
chloroform | 20% (v/v), 2 h, 20°C, 71% loss of activity | Microbulbifer thermotolerans | |
Co2+ | 10 mM, 47% loss of activity | Microbulbifer thermotolerans | |
Cu2+ | 10 mM, 81% loss of activity | Microbulbifer thermotolerans | |
EDTA | 10 mM, 83% loss of activity | Microbulbifer thermotolerans | |
ethanol | 20% (v/v), 2 h, 20°C, 39% loss of activity | Microbulbifer thermotolerans | |
Fe3+ | 10 mM, 81% loss of activity | Microbulbifer thermotolerans | |
Hg2+ | 10 mM, 83% loss of activity | Microbulbifer thermotolerans | |
methanol | 20% (v/v), 2 h, 20°C, 44% loss of activity | Microbulbifer thermotolerans | |
n-butanol | 20% (v/v), 2 h, 20°C, 49% loss of activity | Microbulbifer thermotolerans | |
Ni2+ | 10 mM, 23% loss of activity | Microbulbifer thermotolerans | |
Zn2+ | 10 mM, 82% loss of activity | Microbulbifer thermotolerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
soluble starch | KM-value: 1.08 mg/ml | Microbulbifer thermotolerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | high concentrations of NaCl (0.5, 1, 1.5, 2, 3, and 4 M) slightly decreases the activity | Microbulbifer thermotolerans |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 20% (v/v), 2 h, 20°C, 12% loss of activity | Microbulbifer thermotolerans |
benzene | 20% (v/v), 2 h, 20°C, 7% loss of activity | Microbulbifer thermotolerans |
chloroform | 20% (v/v), 2 h, 20°C, 71% loss of activity | Microbulbifer thermotolerans |
Ethanol | 20% (v/v), 2 h, 20°C, 39% loss of activity | Microbulbifer thermotolerans |
hexane | the enzyme exhibits extreme stability toward hexane. 1% loss of activity after 2 h at 20°C in 20% (v/v) hexane | Microbulbifer thermotolerans |
Methanol | 20% (v/v), 2 h, 20°C, 44% loss of activity | Microbulbifer thermotolerans |
n-Butanol | 20% (v/v), 2 h, 20°C, 49% loss of activity | Microbulbifer thermotolerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Microbulbifer thermotolerans | A0A0A0Q4S7 | - |
- |
Microbulbifer thermotolerans DAU221 | A0A0A0Q4S7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Microbulbifer thermotolerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Microbulbifer thermotolerans | maltotriose + ? | - |
? | |
soluble starch + H2O | - |
Microbulbifer thermotolerans DAU221 | maltotriose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmyA | - |
Microbulbifer thermotolerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
in sodium phosphate buffer (pH 6.0) | Microbulbifer thermotolerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
above 90 % activity until 6 h and 80 % activity until 12 h | Microbulbifer thermotolerans |
40 | - |
the preincubated enzyme maintains above 90% activity until 2 h, 80% activity until 4 h, 70% activity until 8 h, and 60% activity until 12 h | Microbulbifer thermotolerans |
50 | - |
the preincubated enzyme maintains 50% activity until 1 h, 30% activity until 6 h, and 20% activity until 12 h | Microbulbifer thermotolerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.35 | - |
- |
Microbulbifer thermotolerans |