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Literature summary for 3.2.1.118 extracted from

  • Kuroki, G.; Poulton, J.E.
    Isolation and characterization of multiple forms of prunasin hydrolase from black cherry (Prunus serotina Ehrh.) seeds (1987), Arch. Biochem. Biophys., 255, 19-26.
    View publication on PubMed
Search for more literature for 3.2.1.118

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol weak inhibition Prunus serotina
castanospermine PH I and PH IIb, competitive Prunus serotina
DTT weak inhibition Prunus serotina
iodoacetamide weak inhibition Prunus serotina
iodoacetic acid weak inhibition Prunus serotina
Mandelamide weak inhibition Prunus serotina
Mandelic acid weak inhibition Prunus serotina
additional information little or no inhibition by Cu2+, Mg2+, Zn2+, Pb2+, Fe2+, Fe3+, Ag2+, no inhibition by diethyldithiocarbamate, 1,10-phenanthroline, 2,2'-dipyridyl or EDTA Prunus serotina
p-chloromercuriphenylsulfonate weak inhibition Prunus serotina
p-hydroxymandelic acid weak inhibition Prunus serotina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
 PH IIb and PH I, gel filtration, SDS-PAGE Prunus serotina
69500
-
 PH IIa, SDS-PAGE Prunus serotina
140000
-
 PH IIa, gel filtration Prunus serotina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-prunasin + H2O Prunus serotina involved in cyanogenesis from (R)-amygdalin mandelonitrile + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus serotina
-
 Ehrh., black cherry
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein all isoforms Prunus serotina

Purification (Commentary)

Purification (Comment) Organism
PH I and PH II can be completely resolved by hydroxyapatite chromatography Prunus serotina

Reaction

Reaction Comment Organism Reaction ID
(R)-prunasin + H2O = D-glucose + mandelonitrile three isoforms found, PH I, PH IIa, PH IIb Prunus serotina
a

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Prunus serotina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-prunasin + H2O
-
 Prunus serotina D-glucose + mandelonitrile
-
 ?
(R)-prunasin + H2O involved in cyanogenesis from (R)-amygdalin Prunus serotina mandelonitrile + D-glucose
-
 ?
(S)-sambunigrin + H2O i.e. epimer of (R)-prunasin, poor substrate Prunus serotina D-glucose + mandelonitrile
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O very effective substrate Prunus serotina 2-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O poor substrate Prunus serotina 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Prunus serotina 4-nitrophenol + D-glucose
-
 ?
additional information no substrates are (R)-amygdalin, linamarin, (neo)linustatin, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-alpha-D-mannoside, 4-nitrophenyl-beta-D-xyloside and (S)-dhurrin Prunus serotina ?
-
 ?
salicin + H2O very poor substrate Prunus serotina D-glucose + salicyl alcohol
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 69500, SDS-PAGE Prunus serotina
monomer 1 * 68000, SDS-PAGE, PH I and PH IIb Prunus serotina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 all isoforms Prunus serotina

pH Range

pH Minimum pH Maximum Comment Organism
4 7 all isoforms, half maximum activity Prunus serotina

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.09
-
 castanospermine PH IIb, pH 5, 30°C Prunus serotina
0.19
-
 castanospermine PH I, pH 5, 30°C Prunus serotina