Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetate | enzyme A, enzyme B is not affected | Amylomyces rouxii | |
chitosan | substrate inhibition, enzyme B | Amylomyces rouxii | |
Cu2+ | - |
Amylomyces rouxii | |
Hg2+ | - |
Amylomyces rouxii | |
Mn2+ | - |
Amylomyces rouxii | |
Pb2+ | - |
Amylomyces rouxii | |
Sn2+ | - |
Amylomyces rouxii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Amylomyces rouxii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates enzyme B | Amylomyces rouxii | |
K+ | activates enzyme A | Amylomyces rouxii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
enzyme B, gel filtration | Amylomyces rouxii |
79000 | - |
enzyme A, gel filtration | Amylomyces rouxii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | Amylomyces rouxii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amylomyces rouxii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2 forms: A and B | Amylomyces rouxii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.7 | - |
after second Mono Q HR 5/5 chromatography, enzyme A | Amylomyces rouxii |
102.3 | - |
after Superose 6 HR 10/30 chromatography, enzyme B | Amylomyces rouxii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitosan + H2O | - |
Amylomyces rouxii | ? | - |
? | |
chitosan + H2O | hydrolysis of: chitosan extracted from Mucor rouxii cell walls | Amylomyces rouxii | additional information | mixture of dimer, trimer and higher molecular weight oligomers of glucosamine | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme B | Amylomyces rouxii |
55 | - |
enzyme A | Amylomyces rouxii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 5.0, 30 min, stable | Amylomyces rouxii |
40 | - |
pH 5.0, 30 min, stable | Amylomyces rouxii |
60 | - |
30 min, pH 5.0, complete inactivation | Amylomyces rouxii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Amylomyces rouxii |