Cloned (Comment) | Organism |
---|---|
genomic sequence of Csn2 cloned from the DNA library by PCR | Gongronella sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits Csn2 activity at 10 mM | Gongronella sp. | |
EDTA | - |
Gongronella sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Gongronella sp. | |
Cu2+ | stimulates Csn2 at 1 mM | Gongronella sp. | |
Mn2+ | activates | Gongronella sp. | |
Sr2+ | activates | Gongronella sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Gongronella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gongronella sp. | B0LV85 | - |
- |
Gongronella sp. JG-2005 | B0LV85 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Gongronella sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitosan + H2O | 85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan | Gongronella sp. | chitobiose + chitotriose + chitotetraose | - |
? | |
colloidal chitosan + H2O | 85% deacetylated, Csn2 displays stronger hydrolysis capability against colloidal chitosan than soluble chitosan | Gongronella sp. JG-2005 | chitobiose + chitotriose + chitotetraose | - |
? | |
glycol chitosan + H2O | weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan | Gongronella sp. | ? | - |
? | |
glycol chitosan + H2O | weak activity against glycol chitosan, Csn2 displays 4% of the activity for colloidal chitosan | Gongronella sp. JG-2005 | ? | - |
? | |
additional information | does not hydrolyze colloidal chitin and carboxylmethyl cellulose | Gongronella sp. | ? | - |
? | |
additional information | does not hydrolyze colloidal chitin and carboxylmethyl cellulose | Gongronella sp. JG-2005 | ? | - |
? | |
soluble chitosan + H2O | 85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan | Gongronella sp. | ? | - |
? | |
soluble chitosan + H2O | 85% deacetylated, Csn2 displays 14% of the activity for colloidal chitosan | Gongronella sp. JG-2005 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Gongronella sp. |
Synonyms | Comment | Organism |
---|---|---|
Csn2 | - |
Gongronella sp. |
GH-75 chitosanase | - |
Gongronella sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | optimal reaction temperature is between 55°C and 60°C | Gongronella sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | half-lives of Csn2 at 50°C and 55°C are 30 min and 11 min, respectively | Gongronella sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Gongronella sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 5.8 | - |
Gongronella sp. |