Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharophagus degradans |
Crystallization (Comment) | Organism |
---|---|
structure reveals a five-domain architecture, with an additional insertion C+ domain that has significant impact on the domain arrangement of the protein monomer and its dimerization. The participation of domain C+ in substrate binding is supported by reduced substrate inhibition upon introducing W773A, W689A, and F696A substitutions within this domain. In addition to Asp335, residue Glu216 is essential for the catalytic activtiy | Saccharophagus degradans |
Protein Variants | Comment | Organism |
---|---|---|
D215A | 15fold decrease in catalytic efficiency | Saccharophagus degradans |
D335A | complete loss of activtiy | Saccharophagus degradans |
D335A | weak binding of substrate | Saccharophagus degradans |
E216A | complete loss of activtiy | Saccharophagus degradans |
E381A | 330fold decrease in catalytic efficiency | Saccharophagus degradans |
F696A | 3-5fold decrease in kcat value | Saccharophagus degradans |
R331A | 26000fold decrease in catalytic efficiency | Saccharophagus degradans |
W689A | 3-5fold decrease in kcat value | Saccharophagus degradans |
W773A | 3-5fold decrease in kcat value | Saccharophagus degradans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 30% residual activity | Saccharophagus degradans | |
Cd2+ | 1 mM, 40% residual activity | Saccharophagus degradans | |
Cu2+ | 1 mM, no residual activity | Saccharophagus degradans | |
Hg2+ | 1 mM, no residual activity | Saccharophagus degradans | |
additional information | not inhibitory: EDTA at 10 mM | Saccharophagus degradans | |
Ni2+ | 1 mM, 70% residual activity | Saccharophagus degradans | |
Zn2+ | 1 mM, 10% residual activity | Saccharophagus degradans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
birchwood xylan | km vaule of wild-type 43 mg/ml, pH 6.5, 40°C | Saccharophagus degradans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70800 | - |
sedimentation-equilibrium ultracentrifugation | Saccharophagus degradans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharophagus degradans | Q21JW4 | - |
- |
Saccharophagus degradans DSM 17024 | Q21JW4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | - |
Saccharophagus degradans | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | - |
? | |
birchwood xylan + H2O | - |
Saccharophagus degradans DSM 17024 | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | - |
? | |
additional information | the enzyme shows activity toward glucuronoxylan and oligomers thereof with preference toward 4-O-methyl glucopyranosyluronic acid linked to internal xylopyranosyl residues | Saccharophagus degradans | ? | - |
? | |
additional information | the enzyme shows activity toward glucuronoxylan and oligomers thereof with preference toward 4-O-methyl glucopyranosyluronic acid linked to internal xylopyranosyl residues | Saccharophagus degradans DSM 17024 | ? | - |
? | |
oat spelt xylan + H2O | - |
Saccharophagus degradans | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | 1% of the activity with birchwood xylan | ? | |
oat spelt xylan + H2O | - |
Saccharophagus degradans DSM 17024 | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | 1% of the activity with birchwood xylan | ? | |
spruce arabinoxylan + H2O | - |
Saccharophagus degradans | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | 37% of the activity with birchwood xylan | ? | |
spruce arabinoxylan + H2O | - |
Saccharophagus degradans DSM 17024 | 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ? | 37% of the activity with birchwood xylan | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 109992, calculated | Saccharophagus degradans |
Synonyms | Comment | Organism |
---|---|---|
Agu115A | - |
Saccharophagus degradans |
Sde_1755 | - |
Saccharophagus degradans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
half-life 90 min | Saccharophagus degradans |
40 | - |
half-life 60 min | Saccharophagus degradans |
50 | - |
half-life 2 min | Saccharophagus degradans |
70 | - |
2 min, complete loss of activtiy | Saccharophagus degradans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
birchwood xylan | mutant R331A, pH 6.5, 40°C | Saccharophagus degradans | |
0.3 | - |
birchwood xylan | mutant E381A, pH 6.5, 40°C | Saccharophagus degradans | |
1 | - |
birchwood xylan | mutant D215A, pH 6.5, 40°C | Saccharophagus degradans | |
86 | - |
birchwood xylan | mutant F696A, pH 6.5, 40°C | Saccharophagus degradans | |
116 | - |
birchwood xylan | mutant W689A, pH 6.5, 40°C | Saccharophagus degradans | |
129 | - |
birchwood xylan | mutant W773A, pH 6.5, 40°C | Saccharophagus degradans | |
289 | - |
birchwood xylan | wild-type, pH 6.5, 40°C | Saccharophagus degradans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharophagus degradans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | 4°C, 24 h, more than 50% resiudal activity | Saccharophagus degradans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
birchwood xylan | kcat/Km value of wild-type 6.7 ml/s/ml, pH 6.5, 40°C | Saccharophagus degradans |