Literature summary for 3.2.1.15 extracted from

Wang, L.; Rong, W.; He, C.
Two Xanthomonas extracellular polygalacturonases, PghAxc and PghBxc, are regulated by type III secretion regulators HrpX and HrpG and are required for virulence (2008), Mol. Plant Microbe Interact., 21, 555-563.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	expression of genes pghAxc and pghBxc, encoding functional polygalacturonase, are regulated by the type III secretion regulators HrpX and HrpG and the global regulator Clp	Xanthomonas campestris pv. campestris

Application

Application	Comment	Organism
additional information	polygalacturonase genes can be efficiently induced in planta and are required for the full virulence of Xanthomonas campestris pv. campestris to Arabidopsis. Polygalacturonase is secreted via the type II secretion system in an Xps-dependent manner	Xanthomonas campestris pv. campestris

Cloned(Commentary)

Cloned (Comment)	Organism
promoter regions of pghAxc and pghBxc cloned and fused with beta-glucuronidase genes	Xanthomonas campestris pv. campestris

Protein Variants

Protein Variants	Comment	Organism
additional information	mutants pghAxc and pghBxc and double mutant, which show lower hydrolytic activities compared to the wild-type, especially the pghAxc mutant and the double mutant. The wild-type causes distinctive necrosis symptoms compared with the mutants and the double mutant, all of which elicite weak symptoms with a minor wilt in both dip- and spray-inoculation assays. Mutant-complemented strains of both pghAxc and pghBxc are able to elicit the same distinctive necrosis symptoms as the wild-type. No differences between the wild-type and the mutants using the infiltration method	Xanthomonas campestris pv. campestris

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris pv. campestris	-	strains 8004, XCM31, XCM32 and XCM321	-

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Release 2023.1 (January 2023)