Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by EDTA | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
0.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
1.3 | - |
4-nitrophenyl-beta-D-mannopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
1.5 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not activated by the presence of monovalent or divalent cations | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
2 * 57000, SDS-PAGE | Thermoplasma acidophilum |
57070 | - |
subunit, MALDI-TOF mass spectrometry | Thermoplasma acidophilum |
114000 | - |
native enzyme, gel filtration | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q9HIL6 | strain DSM 1728 | - |
Purification (Comment) | Organism |
---|---|
Hi-Trap column chromatography, gel filtration | Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
after 68fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
1.2 | - |
crude extract, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
1.7 | - |
after 68fold purification, using 2-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
1.8 | - |
after 68fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
4.9 | - |
after 68fold purification, using 4-nitrophenyl-beta-D-mannopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
8.5 | - |
after 68fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
82 | - |
after 68fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
113 | - |
after 68fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermoplasma acidophilum | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
additional information | no activity is detected for 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinopyranoside | Thermoplasma acidophilum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57000, SDS-PAGE | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
BglA | - |
Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
4-nitrophenyl-beta-D-mannopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
27 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
141 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
182 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the maximum activity of BglA at 75°C is initially at pH 4.5 but after 1 h at 75°C is at pH 6.0 | Thermoplasma acidophilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6 | the enzyme is unstable below pH 6.0, at pH 6.0 its half-life at 75°C is 28 days but at pH 4.5 is 13 h | Thermoplasma acidophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
4-nitrophenyl-beta-D-mannopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
18 | - |
4-nitrophenyl-beta-D-galactopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
381 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum | |
1068 | - |
4-nitrophenyl-beta-D-fucopyranoside | in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C | Thermoplasma acidophilum |