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Literature summary for 3.2.1.23 extracted from
- Galactooligosaccharide production by a thermostable ?-galactosidase from Sulfolobus solfataricus (2008), World J. Microbiol. Biotechnol., 24, 1553-1558.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? |  |
| additional information | the recombinant enzyme produces galactooligosaccharides from lactose by transgalactosylation. Galactooligosaccharide production increases with increasing lactose concentration, whereas the yield of galactooligosaccharides from lactose is almost constant. The final concentration of galactooligosaccharide is maximal at 80°C | Saccharolobus solfataricus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
assay at | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life: 720 h, thermal deactivation of galactooligosaccharide production | Saccharolobus solfataricus |
| 75 | - |
half-life: 208 h, thermal deactivation of galactooligosaccharide production | Saccharolobus solfataricus |
| 80 | - |
half-life: 77 h, thermal deactivation of galactooligosaccharide production | Saccharolobus solfataricus |
| 85 | - |
half-life: 42 h, thermal deactivation of galactooligosaccharide production | Saccharolobus solfataricus |
| 90 | - |
half-life: 9.3 h, thermal deactivation of galactooligosaccharide production | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
transgalactosylation activity is optimal at pH 6.0 | Saccharolobus solfataricus |
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