Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, 49% inhibition, glucoamylase M1 | Aspergillus phoenicis | |
Mn2+ | - |
Aspergillus phoenicis | |
Pb2+ | glucoamylase M2 | Aspergillus phoenicis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus phoenicis | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glucoamylase M1 contains 18% neutral sugar and 0.77% glucosamine | Aspergillus phoenicis |
Purification (Comment) | Organism |
---|---|
glucoamylase M1 | Aspergillus phoenicis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucoside + H2O | - |
Aspergillus phoenicis | 4-nitrophenol + D-glucose | - |
? | |
amylopectin + H2O | - |
Aspergillus phoenicis | D-glucose + ? | - |
? | |
amylose + H2O | - |
Aspergillus phoenicis | glucose + ? | - |
? | |
dextrin + H2O | - |
Aspergillus phoenicis | ? | - |
? | |
glycogen + H2O | amylose A, n = 25 and amylose B, n = 130 | Aspergillus phoenicis | glucose + ? | - |
? | |
isomaltose + H2O | - |
Aspergillus phoenicis | glucose | - |
? | |
maltose + H2O | - |
Aspergillus phoenicis | 2 glucose | - |
? | |
maltotriose + H2O | - |
Aspergillus phoenicis | maltose + glucose | - |
? | |
phenyl alpha-maltoside + H2O | - |
Aspergillus phoenicis | glucose + phenyl alpha-glucoside | - |
? | |
starch + H2O | soluble | Aspergillus phoenicis | glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, glucoamylase I, SDS-PAGE | Aspergillus phoenicis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
glucoamylase M1, stable | Aspergillus phoenicis |
50 | - |
pH 6.0, 5 min, stable up to | Aspergillus phoenicis |
70 | - |
pH 6.0, 5 min, complete loss of activity | Aspergillus phoenicis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
glucoamylase M1, hydrolysis of soluble starch | Aspergillus phoenicis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
37°C, 1 h, complete loss of activity below | Aspergillus phoenicis |
2.5 | 7.5 | 37°C, 1 h, stable | Aspergillus phoenicis |
9 | - |
37°C, 1 h, complete loss of activity above | Aspergillus phoenicis |