Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging drop vapour diffusion method, 20°C, 0.0015 ml of 20 mg/ml enzyme solution is mixed with 0.0015 ml of reservoir solution containing 10% w/v PEG 5000 monomethyl ether, 186 mM D-glucose, 10 mM DTT, and 1.0 M lithium chloride, in 100 mM MES, pH 6.1, cryoprotection by 20% w/v PEG 6000, 20% v/v 2-methyl-2,4-pentanediol, 2.5 mM CaCl2, 40 mM MES, pH 6.1, X-ray diffraction structure determination and analysis at 3.3 A resolution | Thermoactinomyces vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
gel filtration | Thermoactinomyces vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces vulgaris | - |
- |
- |
Thermoactinomyces vulgaris R-47 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain MV1184 by heat treatment at 56°C for 30 min, two steps of ion exchange chromatography, and gel filtration | Thermoactinomyces vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltooligosaccharides + H2O | high activity | Thermoactinomyces vulgaris | maltooligosaccharides + beta-D-glucose | - |
? | |
maltooligosaccharides + H2O | high activity | Thermoactinomyces vulgaris R-47 | maltooligosaccharides + beta-D-glucose | - |
? | |
starch + H2O | soluble starch, very low activity | Thermoactinomyces vulgaris | starch + beta-D-glucose | - |
? | |
starch + H2O | soluble starch, very low activity | Thermoactinomyces vulgaris R-47 | starch + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermoactinomyces vulgaris |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Thermoactinomyces vulgaris |
glucoamylase | - |
Thermoactinomyces vulgaris |
maltooligosaccharide-metabolizing enzyme | - |
Thermoactinomyces vulgaris |
tGA | - |
Thermoactinomyces vulgaris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermoactinomyces vulgaris | isoelectric focusing | - |
5.7 |