Cloned (Comment) | Organism |
---|---|
gene gla1, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris, phylogenetic analysis | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
maltose | pH 5.0, 70°C | Thermomyces lanuginosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | Q58HN1 | precursor; gene gla1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 94% of activity with amylose | Thermomyces lanuginosus | amylopectin + beta-D-glucose | - |
? | |
amylose + H2O | preferred substrate | Thermomyces lanuginosus | amylose + beta-D-glucose | - |
? | |
glycogen + H2O | 87% of activity with amylose | Thermomyces lanuginosus | glycogen + beta-D-glucose | - |
? | |
maltose + H2O | 14% of activity with amylose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
maltotriose + H2O | 40% of activity with amylose | Thermomyces lanuginosus | maltose + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Thermomyces lanuginosus |
Synonyms | Comment | Organism |
---|---|---|
GA | - |
Thermomyces lanuginosus |
glucoamylase | - |
Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.3 | - |
maltose | pH 5.0, 70°C | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermomyces lanuginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces lanuginosus | isoelectric focusing | - |
3.84 |