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Literature summary for 3.2.1.37 extracted from

  • Ye, Y.; Li, X.; Zhao, J.
    Production and characteristics of a novel xylose- and alkali-tolerant GH 43 beta-xylosidase from Penicillium oxalicum for promoting hemicellulose degradation (2017), Sci. Rep., 7, 11600 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Penicillium oxalicum

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ about 38% residual activity at 5 mM Penicillium oxalicum
Cu2+ about 80% residual activity at 5 mM Penicillium oxalicum
D-xylose about 55% residual activity in the presence of 20 mM xylose Penicillium oxalicum
EDTA about 75% residual activity at 5 mM Penicillium oxalicum
K+ about 77% residual activity at 5 mM Penicillium oxalicum
additional information not inhibited by glucose, arabinose, mannose, and galactose Penicillium oxalicum
Pb2+ about 40% residual activity at 5 mM Penicillium oxalicum
SDS about 42% residual activity at 5 mM Penicillium oxalicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.05
-
 4-nitrophenyl beta-D-xylopyranoside at pH 7.0 and 50°C Penicillium oxalicum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mn2+, Ca2+, Fe3+, Co2+ and Zn2+ have no effects on enzyme activity Penicillium oxalicum

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum S7ZRR2
-
-
Penicillium oxalicum 114-2 S7ZRR2
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Penicillium oxalicum 4-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Penicillium oxalicum 114-2 4-nitrophenol + beta-D-xylopyranose
-
 ?
additional information no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-L-arabinofuranoside, 4-nitrophenyl-D-cellobioside, sugarcane bagasse xylan, corncob xylan, beechwood xylan, glucan, mannan, polygalactose and arabinan Penicillium oxalicum ?
-
 ?
additional information no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-L-arabinofuranoside, 4-nitrophenyl-D-cellobioside, sugarcane bagasse xylan, corncob xylan, beechwood xylan, glucan, mannan, polygalactose and arabinan Penicillium oxalicum 114-2 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Penicillium oxalicum
? x * 38610, calculated from amino acid sequence Penicillium oxalicum

Synonyms

Synonyms Comment Organism
beta-xylosidase
-
 Penicillium oxalicum
xyl43
-
 Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penicillium oxalicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 more than 40% activity between 30 and 60°C Penicillium oxalicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme remains stable up to 50°C after 30 min of incubation but the activity rapidly decreases when the temperature above 50°C Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Penicillium oxalicum

pH Range

pH Minimum pH Maximum Comment Organism
6 9 the enzyme retains 76.35% and 55.85% of catalytic activities at pH 6.0 and 9.0, respectively Penicillium oxalicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 the enzyme is relatively stable in the range of pH 6.0 to 9.0, retaining more than 61% residual activity after 30 min of incubation Penicillium oxalicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
28.09
-
 D-xylose at pH 7.0 and 50°C Penicillium oxalicum