Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 118.4% relative activity | Phialophora sp. CGMCC 3328 | |
2-mercaptoethanol | 129.7% relative activity | Phialophora sp. CGMCC 3328 | |
EDTA | 99.4% relative activity | Phialophora sp. CGMCC 3328 | |
EDTA | 99.7% relative activity | Phialophora sp. CGMCC 3328 |
Application | Comment | Organism |
---|---|---|
industry | textile and detergent industry, combination of CBH6A and EgGH45 | Phialophora sp. CGMCC 3328 |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Phialophora sp. CGMCC 3328 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pb2+ | no remaining activity; no remaining activity | Phialophora sp. CGMCC 3328 | |
SDS | 10 mM, 35% remaining activity; 10 mM, 54% remaining activity | Phialophora sp. CGMCC 3328 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethyl cellulose | 46.2 mg/l and vmax: 17.1 micromol/min/mg, pH 7, 60°C | Phialophora sp. CGMCC 3328 | |
additional information | - |
carboxymethyl cellulose | 71.9 mg/l and vmax: 28.6 micromol/min/mg, pH 7, 65°C | Phialophora sp. CGMCC 3328 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 57.8% relative activity | Phialophora sp. CGMCC 3328 | |
Ca2+ | 85.5% relative activity | Phialophora sp. CGMCC 3328 | |
Co2+ | 69.2% relative activity | Phialophora sp. CGMCC 3328 | |
Co2+ | 85.5% relative activity | Phialophora sp. CGMCC 3328 | |
Cr3+ | 76.7% relative activity | Phialophora sp. CGMCC 3328 | |
Cr3+ | 84.1% relative activity | Phialophora sp. CGMCC 3328 | |
Cu2+ | 116.1% relative activity | Phialophora sp. CGMCC 3328 | |
Cu2+ | 118% relative activity | Phialophora sp. CGMCC 3328 | |
Fe3+ | 114.3% relative activity | Phialophora sp. CGMCC 3328 | |
Fe3+ | 87.5% relative activity | Phialophora sp. CGMCC 3328 | |
K+ | 86.2% relative activity | Phialophora sp. CGMCC 3328 | |
K+ | 96.1% relative activity | Phialophora sp. CGMCC 3328 | |
Li+ | 84.6% relative activity | Phialophora sp. CGMCC 3328 | |
Li+ | 97% relative activity | Phialophora sp. CGMCC 3328 | |
Mg2+ | 108.5% relative activity | Phialophora sp. CGMCC 3328 | |
Mg2+ | 99.8% relative activity | Phialophora sp. CGMCC 3328 | |
Na+ | 97.6% relative activity | Phialophora sp. CGMCC 3328 | |
Na+ | 99.4% relative activity | Phialophora sp. CGMCC 3328 | |
Ni2+ | 100.8% relative activity | Phialophora sp. CGMCC 3328 | |
Ni2+ | 107.1% relative activity | Phialophora sp. CGMCC 3328 | |
Zn2+ | 110% relative activity | Phialophora sp. CGMCC 3328 | |
Zn2+ | 99.9% relative activity | Phialophora sp. CGMCC 3328 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
difference to 56000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE | Phialophora sp. CGMCC 3328 |
36000 | - |
difference to 56000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE | Phialophora sp. CGMCC 3328 |
56000 | - |
difference to 36000 due to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE | Phialophora sp. CGMCC 3328 |
56000 | - |
difference to 36000 duet to posttranslational modification during heterologous expression in Pichia pastoris, SDS-PAGE | Phialophora sp. CGMCC 3328 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phialophora sp. CGMCC 3328 | F2VRY9 | HM003039; G5 CGMCC 3328 | - |
Phialophora sp. CGMCC 3328 | F2VRZ0 | HM003040; G5 CGMCC 3328 | - |
Purification (Comment) | Organism |
---|---|
centrifuged at 12,000 x g for 10 min to remove cell depris, hot shock, ultrafiltration, dialysis, gel filtration | Phialophora sp. CGMCC 3328 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
avicel + H2O | 14% of the activity with carboxymethyl-cellulose | Phialophora sp. CGMCC 3328 | ? | - |
? | |
avicel + H2O | 17.7% of the activity with carboxymethyl-cellulose | Phialophora sp. CGMCC 3328 | ? | - |
? | |
barley glucan + H2O | 120% of the activity with carboxymethyl-cellulose | Phialophora sp. CGMCC 3328 | ? | - |
? | |
barley glucan + H2O | 143% of the activity with carboxymethyl-cellulose | Phialophora sp. CGMCC 3328 | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Phialophora sp. CGMCC 3328 | ? | - |
? | |
filter paper + H2O | 4% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 | Phialophora sp. CGMCC 3328 | ? | - |
? | |
filter paper + H2O | 8% of the activity with carboxymethyl-cellulose. CBH6A and EgGH45, synergic effect, showing the highest efficiency in the ratio at 80:20 | Phialophora sp. CGMCC 3328 | ? | - |
? | |
additional information | no activity against birchwood xylan, 4-nitrophenyl cellobiose, 4-nitrophenyl beta-D-glucoside | Phialophora sp. CGMCC 3328 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cbh6A | gene name, consists of a family 1 CBM and a catalytic domain of glycosyl hydrolase family 6 cellobiohydrolases | Phialophora sp. CGMCC 3328 |
egGH45 | gene name, contains a catalytic domain of family 45 endoglucanases | Phialophora sp. CGMCC 3328 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
50-70, determnined at 30-80°C, pH 6 for 30 min | Phialophora sp. CGMCC 3328 |
65 | - |
50-70, determnined at 30-80°C, pH 7 for 30 min | Phialophora sp. CGMCC 3328 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: 20% relative activity, respectively, 70°C: 70% relative activity | Phialophora sp. CGMCC 3328 |
30 | 70 | 30°C: 30% relative activity, respectively, 70°C: 70% relative activity | Phialophora sp. CGMCC 3328 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
CBH6A and EgGH45, 90% activity for 10 min | Phialophora sp. CGMCC 3328 |
80 | - |
CBH6A and EgGH45, 80% activity for 10 min | Phialophora sp. CGMCC 3328 |
90 | - |
CBH6A and EgGH45, 47% activity for 10 min | Phialophora sp. CGMCC 3328 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | EgGH45, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min | Phialophora sp. CGMCC 3328 |
7 | - |
CBH6A, measured in various buffers at pH 3-11 (McIlvaine, Tris-HCl, glycine-NaOH) at 60°C for 30 min | Phialophora sp. CGMCC 3328 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | pH 3: 20% relative activity, pH 11: 35% relatice activity at 65°C | Phialophora sp. CGMCC 3328 |
3 | 11 | pH 3: 25% relative activity, pH 11: 40% relatice activity at 65°C | Phialophora sp. CGMCC 3328 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 11 | 80-100% acticity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 60°C and pH 6.0 for EgGH45 | Phialophora sp. CGMCC 3328 |
2 | 11 | 80-100% activity, enzymes are preincubated in the buffers without substrate at 37°C for 2 h, and the residual activities are measured at optimal conditions for 30 min, 65°C and pH 7 | Phialophora sp. CGMCC 3328 |