Cloned (Comment) | Organism |
---|---|
expression in Penicillium canescens | Talaromyces verruculosus |
Protein Variants | Comment | Organism |
---|---|---|
N194A | mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability. Slight shift of pH optimum 4.5 for wild-type to 5.0 and 32-35% increase in the specific activity against carboxymethylcellulose and barley beta-glucan | Talaromyces verruculosus |
N19A | mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability but 26% decrease in the specific activity against carboxymethylcellulose and 12% against barley beta-glucan | Talaromyces verruculosus |
N42A | mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability. Slight shift of pH optimum 4.5 for wild-type to 5.0 and 12-13% increase in the specific activity against carboxymethylcellulose and barley beta-glucan | Talaromyces verruculosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Barley beta-glucan | Km value 20 mg/ml for wild-type, 13 mg/ml for mutant N19A, 21 mg/ml for mutant N42A, 22 mg/ml for mutant N194A, respectively, pH 5.0, 40°C | Talaromyces verruculosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces verruculosus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42 | - |
mutant N19A, substrate carboxymethylcellulose, pH 5.0, 40°C | Talaromyces verruculosus |
53 | - |
mutant N19A, substrate barley beta-glucan, pH 5.0, 40°C | Talaromyces verruculosus |
57 | - |
wild-type, substrate carboxymethylcellulose, pH 5.0, 40°C | Talaromyces verruculosus |
60 | - |
wild-type, substrate barley beta-glucan, pH 5.0, 40°C | Talaromyces verruculosus |
64 | - |
mutant N42A, substrate carboxymethylcellulose, pH 5.0, 40°C | Talaromyces verruculosus |
68 | - |
mutant N42A, substrate barley beta-glucan, pH 5.0, 40°C | Talaromyces verruculosus |
75 | - |
mutant N194A, substrate carboxymethylcellulose, pH 5.0, 40°C | Talaromyces verruculosus |
81 | - |
mutant N194A, substrate barley beta-glucan, pH 5.0, 40°C | Talaromyces verruculosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
carboxymethylcellulose + H2O | - |
Talaromyces verruculosus | ? | - |
? | |
milled aspen wood + H2O | - |
Talaromyces verruculosus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel5A | - |
Talaromyces verruculosus |
endoglucanase IIa | - |
Talaromyces verruculosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
127 | - |
Barley beta-glucan | mutant N19A, pH 5.0, 40°C | Talaromyces verruculosus | |
165 | - |
Barley beta-glucan | wild-type, pH 5.0, 40°C | Talaromyces verruculosus | |
227 | - |
Barley beta-glucan | mutant N42A, pH 5.0, 40°C | Talaromyces verruculosus | |
275 | - |
Barley beta-glucan | mutant N194A, pH 5.0, 40°C | Talaromyces verruculosus |