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Literature summary for 3.2.1.52 extracted from

  • Sun, Y.; Zhang, J.; Xiang, J.
    Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda (2018), Gene, 648, 12-20 .
    View publication on PubMed
Search for more literature for 3.2.1.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Palaemon carinicauda

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ about 85% residual activity at 10 mM Palaemon carinicauda
Cu2+ about 87% residual activity at 10 mM Palaemon carinicauda
Hg2+
-
 Palaemon carinicauda

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ about 130% activity at 10 mM Palaemon carinicauda
Mn2+ about 120% activity at 10 mM Palaemon carinicauda
additional information not influenced by Ca2+, K+, Fe3+, Mg2+, and Zn2+ Palaemon carinicauda

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Palaemon carinicauda
-
 N-acetyl-beta-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Palaemon carinicauda A0A2P1BRR8 ridgetail white prawn
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography Palaemon carinicauda

Source Tissue

Source Tissue Comment Organism Textmining
epidermis high expression Palaemon carinicauda
-
hepatopancreas highest expression Palaemon carinicauda
-
intestine
-
 Palaemon carinicauda
-
additional information not detected in heart, gill, eyestalk, nerve or muscle Palaemon carinicauda
-
stomach
-
 Palaemon carinicauda
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
 Palaemon carinicauda 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
chitin + H2O
-
 Palaemon carinicauda N-acetyl-beta-D-glucosamine
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, SDS-PAGE Palaemon carinicauda
? x * 66953, mature peptide, calculated from amino acid sequence Palaemon carinicauda

Synonyms

Synonyms Comment Organism
beta-N-acetylglucosaminidase
-
 Palaemon carinicauda
NAGase
-
 Palaemon carinicauda

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Palaemon carinicauda

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 more than 50% activity between 20 and 50°C Palaemon carinicauda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Palaemon carinicauda

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 more than 50% activity between pH 3.5 and 6.0 Palaemon carinicauda

pI Value

Organism Comment pI Value Maximum pI Value
Palaemon carinicauda calculated from amino acid sequence
-
 4.8

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
10
-
 at pH 5.0 and 40°C Palaemon carinicauda Hg2+

Expression

Organism Comment Expression
Palaemon carinicauda during the molting stages, the enzyme mRNA expression is well-regulated and its expression reaches the highest level at the molting stage E. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the enzyme expression is up-regulated significantly at 6 h and reaches the peak at 12 h up