Cloned (Comment) | Organism |
---|---|
gene abfA encoding alpha-L-arabinofuranosidase homologously expressed in Streptomyces lividans by complementation of mutant IAF10-164 and DNA sequence determined, multicopy vector pIJ702 permits overproduction of the enzyme | Streptomyces lividans |
gene xylB encoding AF, heterologously cloned in Escherichia coli | Butyrivibrio fibrisolvens |
gene, encoding AF, heterologously cloned in Escherichia coli | Aspergillus niger |
gene, encoding AF, heterologously expressed in Escherichia coli JM83 | Thermoclostridium stercorarium |
xynC gene, encoding AF, heterologously expressed in Escherichia coli | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
p-nitrophenyl-alpha-L-arabinofuranoside | - |
Streptomyces lividans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
6 * 69000, SDS-PAGE | Streptomyces lividans |
72490 | - |
calculated from DNA and amino acid sequence analysis | Streptomyces lividans |
380000 | - |
gel filtration | Streptomyces lividans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beet arabinan + H2O | Bacillus subtilis | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Aspergillus niger | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Butyrivibrio fibrisolvens | - |
L-arabinose + ? | - |
? | |
beet arabinan + H2O | Streptomyces sp. | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Clostridium acetobutylicum | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Streptomyces lividans | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Athelia rolfsii | - |
L-arabinose + ? | - |
ir | |
beet arabinan + H2O | Streptomyces purpurascens | - |
L-arabinose + ? | - |
? | |
rye flour arabinoxylan + H2O | Streptomyces lividans | - |
L-arabinofuranose + ? | - |
? | |
wheat-flour arabinoxylan + H2O | Streptomyces lividans | - |
L-arabinofuranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Athelia rolfsii | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Butyrivibrio fibrisolvens | - |
- |
- |
Clostridium acetobutylicum | - |
- |
- |
Dichomitus squalens | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Streptomyces lividans | - |
mutant IAF10-164 | - |
Streptomyces lividans | - |
66strain 1326 | - |
Streptomyces lividans 66 1326 | - |
66strain 1326 | - |
Streptomyces purpurascens | - |
- |
- |
Streptomyces sp. | - |
no. 17-1 | - |
Streptomyces sp. 17-1 | - |
no. 17-1 | - |
Thermoclostridium stercorarium | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
- |
Trichoderma reesei |
- |
Streptomyces lividans |
- |
Athelia rolfsii |
- |
Dichomitus squalens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
153 | - |
- |
Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arabinoxylobiose + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + xylobiose | - |
? | |
arabinoxylobiose + H2O | - |
Streptomyces lividans | L-arabinose + xylobiose | - |
? | |
arabinoxylobiose + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + xylobiose | - |
? | |
beet arabinan + H2O | - |
Bacillus subtilis | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + ? | - |
? | |
beet arabinan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Clostridium acetobutylicum | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Athelia rolfsii | L-arabinose + ? | - |
ir | |
beet arabinan + H2O | - |
Streptomyces purpurascens | L-arabinose + ? | - |
? | |
L-arabinan + H2O | - |
Bacillus subtilis | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinan + H2O | - |
Aspergillus niger | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinan + H2O | - |
Clostridium acetobutylicum | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinan + H2O | - |
Streptomyces lividans | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinan + H2O | - |
Streptomyces purpurascens | (1,5-alpha-L-arabinose)25 + ? | - |
? | |
L-arabinogalactan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Dichomitus squalens | L-arabinose + ? | - |
? | |
L-arabinogalactan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
? | |
L-arabinoxylan + H2O | - |
Aspergillus niger | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces sp. | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Trichoderma reesei | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Thermoclostridium stercorarium | L-arabinose + ? | - |
ir | |
L-arabinoxylan + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + ? | - |
ir | |
L-arabinoxylohexaose + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
? | |
L-arabinoxylopentaose + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
? | |
L-arabinoxylotetraose + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
? | |
L-arabinoxylotetraose + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
? | |
L-arabinoxylotriose + H2O | - |
Streptomyces lividans | L-arabinose + ? | - |
? | |
L-arabinoxylotriose + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + ? | - |
? | |
additional information | - |
Bacillus subtilis | ? | - |
? | |
additional information | - |
Aspergillus niger | ? | - |
? | |
additional information | - |
Butyrivibrio fibrisolvens | ? | - |
? | |
additional information | - |
Streptomyces sp. | ? | - |
? | |
additional information | - |
Clostridium acetobutylicum | ? | - |
? | |
additional information | no activity on p-nitrophenyl glycosides like alpha-L-arabinopyranose, beta-D-glucose, beta-D-galactopyranose, beta-D-cellobiose, alpha-L-mannopyranose, oat spelts xylan, xylan or arabinogalactan | Streptomyces lividans | ? | - |
? | |
additional information | inactive towards arabinan, arabinoxylan and arabinogalactan | Streptomyces purpurascens | ? | - |
? | |
additional information | - |
Streptomyces sp. 17-1 | ? | - |
? | |
additional information | no activity on p-nitrophenyl glycosides like alpha-L-arabinopyranose, beta-D-glucose, beta-D-galactopyranose, beta-D-cellobiose, alpha-L-mannopyranose, oat spelts xylan, xylan or arabinogalactan | Streptomyces lividans 66 1326 | ? | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | p-nitrophenol + L-arabinose | - |
? | |
p-nitrophenyl-alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | p-nitrophenol + L-arabinose | - |
? | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Bacillus subtilis | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus niger | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Pseudomonas fluorescens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Butyrivibrio fibrisolvens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Clostridium acetobutylicum | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Thermoclostridium stercorarium | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Athelia rolfsii | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces purpurascens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Dichomitus squalens | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces sp. 17-1 | L-arabinose + phenol | - |
ir | |
phenyl alpha-L-arabinofuranoside + H2O | - |
Streptomyces lividans 66 1326 | L-arabinose + phenol | - |
ir | |
rye flour arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinofuranose + ? | - |
? | |
wheat-flour arabinoxylan + H2O | - |
Streptomyces lividans | L-arabinofuranose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 69000, SDS-PAGE | Streptomyces lividans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus subtilis |
60 | - |
- |
Aspergillus niger |
60 | - |
- |
Trichoderma reesei |
60 | - |
- |
Streptomyces lividans |
60 | - |
- |
Dichomitus squalens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
half-life 12 min | Streptomyces lividans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Butyrivibrio fibrisolvens |
6 | - |
- |
Streptomyces sp. |
6 | - |
- |
Streptomyces lividans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
stable for 12 h at 4°C | Streptomyces lividans |