Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(lambdaDE3) cells | Geobacillus vulcani |
Protein Variants | Comment | Organism |
---|---|---|
L307S | the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme | Geobacillus vulcani |
Q90H/L307S | the mutant shows almost 2.5fold increased activity and exhibits higher thermal stability but lower alkaline stability compared to the wild type enzyme | Geobacillus vulcani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus vulcani | - |
- |
- |
Geobacillus vulcani GS90 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tag nickel affinity column chromatography | Geobacillus vulcani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.066 | - |
wild type enzyme, at pH 5.0 and 70°C | Geobacillus vulcani |
142.024 | - |
mutant enzyme Q90H/L307S, at pH 5.0 and 70°C | Geobacillus vulcani |
160.991 | - |
mutant enzyme L307S, at pH 5.0 and 70°C | Geobacillus vulcani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus vulcani | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Geobacillus vulcani GS90 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Geobacillus vulcani |
Synonyms | Comment | Organism |
---|---|---|
ABF | - |
Geobacillus vulcani |
alpha-L-arabinofuranosidase | - |
Geobacillus vulcani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus vulcani |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 72 | the wild type enzyme retains about 20% activity after 100 min at 70°C, about 20% activity after 40 min at 71°C, and less than 5% activity after 30 min at 72°C | Geobacillus vulcani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Geobacillus vulcani |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.6 | the wild type enzyme retains about 20% activity after 100 min at pH 5.0 and 70°C and about 30% activity after 80 min at pH 9.6 and 70°C | Geobacillus vulcani |