Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cis-aconitic acid | more than 2fold stimulation only for phlorizin activity | Macaca radiata | |
Citric acid | highest stimulation at 50 mM and 30°C only for phlorizin activity | Macaca radiata | |
isocitric acid | 1.7fold stimulation only for phlorizin activity | Macaca radiata | |
L-ascorbic acid | 1.6fold stimulation only for phlorizin activity | Macaca radiata | |
L-Tartaric acid | most stimulating effect only for phlorizin activity | Macaca radiata | |
Pyruvic acid | 0.5fold stimulation only for phlorizin activity | Macaca radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutaric acid | 50% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM | Macaca radiata | |
Maleic acid | 55% inhibition for the tartaric acid-stimulated phlorizin hydrolase at 20 mM | Macaca radiata | |
NaCl | 30% inhibition of the tartaric acid-stimulated phlorizin hydrolase at 20 mM | Macaca radiata | |
oxalacetic acid | 50% inhibition of the tartaric acid-stimualted phlorizin hydrolase at 20 mM | Macaca radiata | |
oxalic acid | 47% inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM | Macaca radiata | |
Pyruvic acid | strongly inhibitory for the tartaric acid-stimulated phlorizin hydrolase | Macaca radiata | |
SO42- | strong inhibition of the tartaric acid-activated phlorizin hydrolase at 20 mM | Macaca radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | Macaca radiata | - |
D-galactose + beta-D-glucose | - |
? | |
phlorizin + H2O | Macaca radiata | - |
phloretin + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca radiata | - |
monkey | - |
Purification (Comment) | Organism |
---|---|
highly purified | Macaca radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | small intestine | Macaca radiata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | 6 | lactase activity | Macaca radiata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-galactopyranosyl-D-glucopyranoside + H2O | - |
Macaca radiata | D-galactose + beta-D-glucose | - |
? | |
phlorizin + H2O | - |
Macaca radiata | phloretin + beta-D-glucose | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 5.6 | phlorizin activity in acetate buffer | Macaca radiata |
5.6 | - |
phlorizin activity at pH 3.3-3.5 in citrate buffer | Macaca radiata |