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Literature summary for 3.2.1.8 extracted from
- Biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of Gryllotalpa orientalis, Cellulosimicrobium sp. HY-12 (2007), Antonie van Leeuwenhoek, 93, 437-442.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM | Cellulosimicrobium sp. |  |
| Co2+ | 10 mM | Cellulosimicrobium sp. | |
| Cu2+ | 10 mM | Cellulosimicrobium sp. | |
| EDTA | - |
Cellulosimicrobium sp. | |
| Fe2+ | 10 mM | Cellulosimicrobium sp. | |
| additional information | 1 mM dithiothreitol has no effect on the enzyme activity | Cellulosimicrobium sp. | |
| N-bromosuccinimide | - |
Cellulosimicrobium sp. | |
| Sodium azide | - |
Cellulosimicrobium sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
mature enzyme, SDS-PAGE | Cellulosimicrobium sp. |
| 42930 | - |
pre-mature enzyme, deduced from amino acid sequence | Cellulosimicrobium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulosimicrobium sp. | - |
- |
- |
| Cellulosimicrobium sp. HY-12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiPrep 16/10 DEAE FF chromatography, HiPrep 16/10 CM FF column chromatography, and Mono Q resin column chromatography | Cellulosimicrobium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beechwood xylan + H2O | - |
Cellulosimicrobium sp. | ? | - |
? | |
| birchwood xylan + H2O | - |
Cellulosimicrobium sp. | ? | - |
? | |
| additional information | can not decompose carboxymethyl cellulose or xylobiose | Cellulosimicrobium sp. | ? | - |
? | |
| additional information | can not decompose carboxymethyl cellulose or xylobiose | Cellulosimicrobium sp. HY-12 | ? | - |
? | |
| oat spelt xylan + H2O | - |
Cellulosimicrobium sp. | ? | - |
? | |
| oat spelt xylan + H2O | - |
Cellulosimicrobium sp. HY-12 | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Cellulosimicrobium sp. | p-nitrophenol + beta-D-cellobiose | weak reaction | ? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellulosimicrobium sp. | p-nitrophenol + beta-D-xylopyranose | weak reaction | ? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellulosimicrobium sp. HY-12 | p-nitrophenol + beta-D-xylopyranose | weak reaction | ? | |
| xylotetraose + H2O | - |
Cellulosimicrobium sp. | ? | - |
? | |
| xylotetraose + H2O | - |
Cellulosimicrobium sp. HY-12 | ? | - |
? | |
| xylotriose + H2O | - |
Cellulosimicrobium sp. | ? | - |
? | |
| xylotriose + H2O | - |
Cellulosimicrobium sp. HY-12 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XYLA | - |
Cellulosimicrobium sp. |
| xylanase | - |
Cellulosimicrobium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Cellulosimicrobium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Cellulosimicrobium sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | retains over 80% of its original activity in the range pH 4.5-7.0 | Cellulosimicrobium sp. |
| 8 | - |
significantly inactivated at pHs over 8.0 | Cellulosimicrobium sp. |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Cellulosimicrobium sp. | mature enzyme, isoelectric focusing | - |
5.16 |
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