Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | enhances the activity about 1.3- and 1.6fold at the concentrations of 1 and 10 mM, respectively | Streptomyces sp. S27 | |
additional information | expression level of XynBS27 increases with induction time | Streptomyces sp. S27 |
Application | Comment | Organism |
---|---|---|
industry | XynBS27 has several advantageous properties such as high specific activity, good activity over broad pH range, excellent pH stability, and being suitable for xylobiose production. These significant characteristics suggest that XynBS27 may be a good candidate in industrial application | Streptomyces sp. S27 |
Cloned (Comment) | Organism |
---|---|
into the pPIC9 vector and overexpressed in Pichia pastoris GS115 | Streptomyces sp. S27 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strongly inhibits by 69.1% even at the concentration of 1 mM | Streptomyces sp. S27 | |
Ca2+ | 10 mM reduces the enzyme activity by 9.7% | Streptomyces sp. S27 | |
Co2+ | 10 mM reduces the enzyme activity by 26.1% | Streptomyces sp. S27 | |
Cu2+ | 10 mM reduces the enzyme activity by 49.6% | Streptomyces sp. S27 | |
Fe2+ | 10 mM reduces the enzyme activity by 31.0% | Streptomyces sp. S27 | |
Fe3+ | 10 mM reduces the enzyme activity by 11.3% | Streptomyces sp. S27 | |
Hg2+ | 1 mM completely inhibits | Streptomyces sp. S27 | |
K+ | 10 mM reduces the enzyme activity by 11.1% | Streptomyces sp. S27 | |
Li+ | 10 mM reduces the enzyme activity by 16.1% | Streptomyces sp. S27 | |
additional information | EDTA, Ni2+, Zn2+ and Mg2+ have no effect on activity | Streptomyces sp. S27 | |
Na+ | 10 mM reduces the enzyme activity by 9.8% | Streptomyces sp. S27 | |
Pb2+ | 10 mM reduces the enzyme activity by 32.9% | Streptomyces sp. S27 | |
SDS | 10 mM reduces the enzyme activity by 59.2% | Streptomyces sp. S27 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | 10 mM enhances the enzyme activity by 16.3% | Streptomyces sp. S27 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
sequence analysis | Streptomyces sp. S27 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. S27 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant XynBS27 purified to electrophoretic homogeneity by anion exchange chromatography | Streptomyces sp. S27 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3272 | - |
purified enzyme | Streptomyces sp. S27 |
Storage Stability | Organism |
---|---|
4°C | Streptomyces sp. S27 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | main hydrolysis product of xylan is xylobiose (more than 75%) | Streptomyces sp. S27 | D-xylose + xylobiose + xylotriose + ? | - |
? | |
oat spelt xylan + H2O | main hydrolysis product of xylan is xylobiose (more than 75%) | Streptomyces sp. S27 | xylobiose + D-xylose + xylotriose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Streptomyces sp. S27 |
XynBS27 | - |
Streptomyces sp. S27 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Streptomyces sp. S27 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1737 | - |
birchwood xylan | - |
Streptomyces sp. S27 | |
3475 | - |
oat spelt xylan | - |
Streptomyces sp. S27 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Streptomyces sp. S27 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | XynBS27 shows good pH stability and retains more than 80% of the maximum activity after incubation at 37°C for 1 h. Between 5-8 retains over 65% of its activity | Streptomyces sp. S27 |