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Literature summary for 3.2.1.8 extracted from

  • Chen, L.L.; Zhang, M.; Zhang, D.H.; Chen, X.L.; Sun, C.Y.; Zhou, B.C.; Zhang, Y.Z.
    Purification and enzymatic characterization of two beta-endoxylanases from Trichoderma sp. K9301 and their actions in xylooligosaccharide production (2009), Biores. Technol., 100, 5230-5236.
    View publication on PubMed
Search for more literature for 3.2.1.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.47
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX1, beechwood xylan Trichoderma sp.
8.33
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX2, beechwood xylan Trichoderma sp.
8.87
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX1, birchwood xylan Trichoderma sp.
11.7
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX2, birchwood xylan Trichoderma sp.
20
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX1, oat spelt xylan Trichoderma sp.
22.2
-
 beta-1,4-xylan pH 5.6, 50°C, isozyme EX2, oat spelt xylan Trichoderma sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the endoxylanases are secreted Trichoderma sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20100
-
 x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE Trichoderma sp.
30100
-
 x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE Trichoderma sp.

Organism

Organism UniProt Comment Textmining
Trichoderma sp.
-
 two acidic beta-endoxylanases EX1 and EX2
-
Trichoderma sp. K9301
-
 two acidic beta-endoxylanases EX1 and EX2
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes EX1 and EX2 are purified from crude extract of the strain K9301 in solid fermentation by anion exchange chromatography and gel filtration to homogeneity Trichoderma sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 2836 U/g dry weight, secreted enzymes Trichoderma sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O isozyme EX1 exhibits better affinity but lower hydrolytic efficiency than isozyme EX2 to xylans from beechwood, birchwood, and oat-spelt Trichoderma sp. xylo-oligosaccharides product analysis by HPLC, overview ?
beta-1,4-xylan + H2O isozyme EX1 exhibits better affinity but lower hydrolytic efficiency than isozyme EX2 to xylans from beechwood, birchwood, and oat-spelt Trichoderma sp. K9301 xylo-oligosaccharides product analysis by HPLC, overview ?
xylo-oligosaccharide + H2O substrates from corn cobs Trichoderma sp. ?
-
 ?
xylo-oligosaccharide + H2O substrates from corn cobs Trichoderma sp. K9301 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30100, isozyme EX1, SDS-PAGE, x * 20100, isozyme EX2, SDS-PAGE Trichoderma sp.

Synonyms

Synonyms Comment Organism
beta-endoxylanase
-
 Trichoderma sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 isozyme EX2 Trichoderma sp.
70
-
 isozyme EX1 Trichoderma sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range, isozyme EX2 Trichoderma sp.
30 90 activity range, isozyme EX1 Trichoderma sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 purified isozyme EX1, stable at temperatures below 40°C, retains 50% activity after incubation at 50°C for 30 h, but it loses its activity rapidly at 60°C for 1 h Trichoderma sp.
50 60 purified EX2, 48 h, 50% activity remaining Trichoderma sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Trichoderma sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isozyme EX2 Trichoderma sp.
5.5
-
 isozyme EX1 Trichoderma sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 9 activity range of EX1 and EX2, profiles, overview Trichoderma sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 stability profile of EX1 and EX2, highly to completely stable at pH 4.0 to pH 9.0, inactivation at pH 3.0 and pH 10.0, overview Trichoderma sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Trichoderma sp.