Cloned (Comment) | Organism |
---|---|
gene xynA, DNA and amino acid sequence determination and analysis, sequence comparisons, expression in of His-tagged XynA in Escherichia coli strain DH10B and JM109 | Bacillus pumilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the activity of the xylanase is not significantly affected by metal ions and EDTA | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
x * 23000, SDS-PAGE | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | Q5EFR9 | gene XynA | - |
Bacillus pumilus ARA | Q5EFR9 | gene XynA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged XynA from Escherichia coli by nickel affinity and anion exchange chromatography | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the main product of hydrolysis by XynA are xylobiose, xylotriose, xylotetraose and other xylooligosaccharides, only trace amount of xylose appear | Bacillus pumilus | ? | - |
? | |
additional information | the main product of hydrolysis by XynA are xylobiose, xylotriose, xylotetraose and other xylooligosaccharides, only trace amount of xylose appear | Bacillus pumilus ARA | ? | - |
? | |
oat spelt xylan + H2O | - |
Bacillus pumilus | ? | - |
? | |
oat spelt xylan + H2O | - |
Bacillus pumilus ARA | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23000, SDS-PAGE | Bacillus pumilus |
Synonyms | Comment | Organism |
---|---|---|
More | a glycoside hydrolase family 11 endoxylanase | Bacillus pumilus |
XynA | - |
Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant enzyme | Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
purified recombinant enzyme, 2 h, stable | Bacillus pumilus |
60 | - |
purified recombinant enzyme, 1 h, over 50% activity remaining | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
recombinant enzyme | Bacillus pumilus |